Composition for modulating sterols in yeast

Details Composition for modulating sterols in yeast Composition for modulating sterols in yeast

1994-05-10

1996-01-02

Moody, Patrica R.

Chemistry: molecular biology and microbiology

Vector, per se

530371, 536 232, 536 2374, 435 52, 435 53, 435 711, 435171, 4352523, 435233, 935 60, 935 69, C12N 1561, C12N 1581, C12N 119

Patent

active

054808057

ABSTRACT:
A structural gene encoding a polypeptide with .DELTA.8-7 sterol isomerase activity is disclosed, Recombinant DNA molecules useful for transforming yeast and mutant yeast transformed with such recombinant DNA molecules are also disclosed. The structural gene is useful for modulating the accumulation of sterols in yeast comprising increasing the expression level of a structural gene encoding a polypeptide having .DELTA.8-7 sterol isomerase activity in a mutant yeast having single or double defects in the expression of sterol biosynthetic enzymes is provided.

REFERENCES:
Ashman et al. 1991 (week of 16 Aug.) Lipids 26 (8):628-632.
Arthington et al. 1991 (30 Oct.) Gene 107(1):173-174.
Rose 1987 In Methods in Enzymology 152; 481-504.
Bard et al. 1990 Inform 1(4):324-325 (Abstract, EE5).

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