Composition for lipase assay

Details Composition for lipase assay Composition for lipase assay

1988-09-09

1989-07-04

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 4, 435 18, 435 19, 435 25, 435 26, 435 21, C12Q 148, C12Q 100, C12Q 134, C12Q 144

Patent

active

048450286

ABSTRACT:
A composition and method for lipase assay, comprising the use of an aqueous solution of a 1,2-diglyceride of a higher fatty acid, and a nonionic surface active agent. The higher fatty acid is a higher fatty acid of 8 or more and preferably 12 or more carbons, most preferably a higher unsaturated fatty acid of more than 16 carbons. The concentration of 1,2-diglyceride is more than 0.5 g per liter of solution. The nonionic surface active agent is a polyoxyethylene-type nonionic surface active agent, a polyhydric-alcohol-type nonionic surface active agent or a block-polymer-type nonionic surface active agent, whose concentration is more than 0.1% by weight in the composition and which has an HLB more than 10. The composition preferably contains 0.5-10 g of 1,2-diglyceride and 10-50 g of nonionic surface active agent per liter of solution.

REFERENCES:
patent: 4241178 (1980-12-01), Esders et al.
patent: 4491631 (1985-01-01), Imamura et al.
patent: 4555483 (1985-11-01), LiMuti et al.
Chem. Abs., 93:233,542p, (1980).
Garland et al., Nature, 196:987-988, Oct. 6, 1982.
Hagen et al., Can. J. Biochem. Phys., 40:1129-1139, (1962).

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