Composition for introducing nucleic acid complexes into higher e

Details Composition for introducing nucleic acid complexes into higher e Composition for introducing nucleic acid complexes into higher e

1995-05-25

2000-02-08

Stanton, Brian R.

Chemistry: molecular biology and microbiology

Vector, per se

435455, 435456, 514 44, 514 2, 514 12, 536 231, 530350, 530300, 530325, 5303875, 53038821, 53038822, C12N 1563, C12N 1500, C12Q 170, C07H 2104

Patent

active

060227354

ABSTRACT:
A composition for the transfection of higher eucaryotic cells, comprising complexes of nucleic acid, a substance having an affinity for nucleic acid and optionally an internalizing factor, contains an endosomolytic agent, e.g. a virus or virus component, which may be conjugated. The endosomolytic agent, which is optionally part of the nucleic acid complex, is internalized into the cells together with the complex and releases the contents of the endosomes into the cytoplasm, thereby increasing the gene transfer capacity. Pharmaceutical preparations, transfection kits and methods for introducing nucleic acid into higher eucaryotic cells by treating the cells with the composition are also disclosed.

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