Composition for external application containing a...

Details Composition for external application containing a... Composition for external application containing a...

1999-01-12

2001-06-26

Saucier, Sandra E. (Department: 1651)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

C424S401000, C435S101000, C435S912000

Reexamination Certificate

active

06251877

ABSTRACT:

BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a process for liquid cultivating
Schizophyllum commune
Fr. for isolation of &bgr;-1,6-branched-&bgr;-1,3-glucan and a composition for external application containing &bgr;-1,6-branched-&bgr;-1,3-glucan obtained by the same.
2. Description of the Prior Art
The skin has a protection function against the surrounding environments such as change of temperature or humidity, ultraviolet, pollution materials. However, the normal function of the skin may be depressed by the excessive physical or chemical stimulation and stresses, or nutrient deficiency, causing skin-aging and skin-damage. So, in the cosmetic filed, for keeping the skin beautiful and healthy by preventing this hypergasia, physiological active materials derived from animals, plants or microorganisms have been used as a cosmetic ingredient.
For example, it has been reported that extracts of fungi have a moisture retention ability, inhibition ability of melanin-formation and screening ability of ultraviolet and thereby can be incorporated in a cosmetic composition to impart skin-whitening effect or anti-oxidative action. In particular, an extract of
Schizophyllum commune
Fr. to impact excellent skin whitening activity and moisture retention effect has been disclosed in JP-5-286843A.
However, its active component has not been revealed yet. That is to say, what component of the extract of
Schizophyllum commune
Fr. can impart effective skin action needs further research to be revealed. Such a further research may include improvement in method for effective and pure isolation of active components.
Therefore, the present inventors have conducted extensive studies in order to reveal active components of the extract of
Schizophyllum commune
Fr. to impact effective skin action. As a result, they found that &bgr;-1,6-branched-&bgr;-1,3-glucan obtained by liquid cultivation of
Schizophyllum commune
Fr. can inhibit melanin-formation, promote biosynthesis of skin-cells and collagen, and cure burn caused by excessive sun exposure.
Further, they found that yield of &bgr;-1,6-branched-&bgr;-1,3-glucan may be increased by adding an activated charcoal or glucose into the liquid medium. They also found that the yield of &bgr;-1,6-branched-&bgr;-1,3-glucan can be more increased by adding both activated charcoal and glucose into the liquid medium.
SUMMARY OF THE INVENTION
Thus, an object of the invention is to provide a process for liquid cultivating
Schizophyllum commune
Fr. for isolation of &bgr;-1,6-branched-&bgr;-1,3-glucan.
Further, other object of the invention is to provide a composition for external application containing &bgr;-1,6-branched-&bgr;-1,3-glucan obtained by the present process as an active ingredient, which can defer skin aging, impart skin whitening effect and cure skin damage effectively.
The object of the present invention can be accomplished by a process characterized in that it comprises steps of
(1) preparing mycelia of
Schizophyllum commune
Fr.;
(2) cultivating the mycelia on a liquid medium with pH of 4.0~8.5 comprising 1~10% by weight of glucose, 0.1~1% by weight of yeast extract, 0.05~0.5% by weight of (NH
4
)
2
HPO
4
or (NH
4
)
2
SO
4
, 0.05~0.5% by weight of KH
2
PO
4
and 0.005~0.2% by weight of MgSO
4
·7H
2
O;
(3) isolating &bgr;-1,6-branched-&bgr;-1,3-glucan from the liquid medium; and
(4) purifying &bgr;-1,6-branched-&bgr;-1,3-glucan.
The present invention also provides a cultivation process further comprising a step of adding an activated charcoal into the liquid medium during the step(2).
The present invention also provides a cultivation process further comprising a step of adding a glucose into the liquid medium during the step(2).
The present invention also provides a cultivation process further comprising a step of adding both activated charcoal and glucose into the liquid medium during the step(2).
Further, the present invention provides a composition for external application characterized in that it contains &bgr;-1,6-branched-&bgr;-1,3-glucan obtained by the present process in an amount 0.00001~10% by weight, based on a total weight of the composition.


REFERENCES:
patent: 3898959 (1975-08-01), Chen et al.
patent: 4098661 (1978-07-01), Kikumoto et al.
patent: 4833131 (1989-05-01), Williams et al.
patent: 4954440 (1990-09-01), Johal et al.
patent: 5320849 (1994-06-01), Hagiwara et al.
patent: 5641643 (1997-06-01), Tanaka et al.
patent: 5-286843 (1993-02-01), None
patent: 67012000 (1993-08-01), None
patent: 5-286843 (1993-11-01), None
patent: WO 9840082 (1998-09-01), None
Eggensperger et al. “Multiactive polysaccharides. Part. 1. Fungi extracts”, SOFW Journal. 1997, vol. 123, No. 8, pp. 542-546.*
Mansell et al. “Polysaccharides in Skin care”. Cosmetics and Toiletries Magazine. Sep. 1994, vol. 109, pp. 67-72.*
Eggensperger et al., SOFW Journal . (1997), 123 (8), pp. 542-546.*
Gura et al., DECHEMA Biotechnology Conferences 5, 1992, pp. 569-572.*
Steiner et al. DECHEMA Biotechnology Conferences 1, 1988, pp. 149-154.*
Prokop et al. Experimental Mycology (1992), 16, pp. 197-206.

Affiliated with

Also associated with

Rating

Composition for external application containing a... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Composition for external application containing a..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Composition for external application containing a... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2449909