Composition for determination of chloride ion

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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435 791, 536 41, 536 178, C12Q 140

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active

054707153

ABSTRACT:
A composition for determination of chloride ion, comprising a maltooligosaccharide derivative possessing modified or non-modified non-reducing terminal and modified reducing terminal, a metal chelating agent, .alpha.-amylase and an adjuvant enzyme, provided that the adjuvant enzyme may not be contained when the maltooligosaccharide derivative possesses reducing terminal with a modifying group bound in .alpha.-type, which does not require maintenance and control of special instruments and special treatment of waste liquors, affords good analytic efficiency, determination precision and linearity, and is capable of suppressing rise of reagent blank and giving accurate determination values from test samples showing high values.

REFERENCES:
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patent: 4963479 (1990-10-01), Chavez
patent: 5011923 (1991-04-01), Ono
patent: 5068182 (1991-11-01), Schmidt
Ono, T., A New Enzymatic Assay of Chloride In Serum, Clin Chem 34/3 (1988) pp. 552-553.
Yamashita H., Substrate Selective Activation of Histidine Modified Porcine . . . J Biochem 110 (1991) pp. 605-607.

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