Composition for delivery of a mitochondrial genome to a cell

Details Composition for delivery of a mitochondrial genome to a cell Composition for delivery of a mitochondrial genome to a cell

2007-10-09

2007-10-09

Crouch, Deborah (Department: 1632)

Chemistry: molecular biology and microbiology

Vector, per se

C435S455000

Reexamination Certificate

active

10485407

ABSTRACT:
The invention provides composition and methods for delivering a composition according to the invention comprising a wild-type (wt) mitochondrial DNA (mtDNA) molecule to a mammalian cell. The wt-mtDNA molecule is a functional mtDNA molecule that includes the entire mitochondrial genome. The wt-mtDNA molecule in the composition of the invention has a mitochondrial leader sequence (MLS) peptide attached to facilitate the uptake of the mtDNA molecule into a mitochondrion of the mammalian cell. The delivery of the wt-mtDNA/MLS peptide complex is used as a general replenishment therapy for conditions attributed to mtDNA defects, independent of the specific defect in the mtDNA.

REFERENCES:
patent: 5939318 (1999-08-01), Miller et al.
patent: 6090619 (2000-07-01), Weissig et al.
patent: 6171863 (2001-01-01), Weissig et al.
patent: 6627618 (2003-09-01), Weissig et al.
Weissig et al. Binding and Release of DNA-Peptide Conjugates by Cationic Mitochondriotropic Vesicles (DQAsomes). Proceed. International Symp. Controlled Release Bioactive Materials. 2001, vol. 28, pp. 850-851.
Seibel et al. Transfection of Mitochondria: Strategy Towards a Gene Therapy of Mitochondria DNA Diseases. Nucleic Acids Res. 1995, vol. 23, pp. 10-17.
Weissig, D'Souza and Torchilin. DQAsomes/DNA Complexes Release DNA Upon Contact With Isolated Mouse Liver Mitochondria. J. Controlled Release. 2001, vol. 75, pp. 401-408.
Chinnery et al. Epidemiology and Treatment of Mitochondrial Disorders. American J. Medical Genetics (Semin. Med. Genet.). 2001, vol. 106, pp. 94-101.
Torchilin et al. Intracellular Targets for DNA Delivery: Nuclei and Mitochondria. Somatic Cell Molec. Genetics. Nov. 2002, vol. 27, pp. 49-64.
Weissig et al., “Lipofection with DQAsomes: Mitochondria-Specific?” Proceed. Int'l. Symp. Control. Rel. Bioact. Mater., 25 (1998) Controlled Release Society, Inc., pp. 180-181.
Weissig, et al., “DQAsomes: A Novel Potential Drug and Gene Delivery System Made from Dequalinium”, Pharmaceutical Resrarch, vol. 15, No. 2, 1998, pp. 334-337.
Weiss, et al., “Dequalinium, a topical antimicrobial agent, displays anticarcinoma activity based on selective mitochondrial Accumulation”, Proc. Natl. Acad. Sci. USA, vol. 84, pp. 5444-5448, Aug. 1987, Medical Sciences.
Zhuo, et al., Inhibition and Photoinactivation of the Bovine Heart Mitochondrial F1-ATPase By The Cytotoxic Agent, Dequalinium, vol. 152, No. 3, 1988, pp. 968-972.

Affiliated with

Also associated with

Rating

Composition for delivery of a mitochondrial genome to a cell does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Composition for delivery of a mitochondrial genome to a cell, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Composition for delivery of a mitochondrial genome to a cell will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3826091