Composition for cell-free protein synthesis

Drug – bio-affecting and body treating compositions – Anti-perspirants or perspiration deodorants – Aluminum compound containing

Reexamination Certificate

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C435S069100, C435S183000, C435S252800, C435S325000, C424S184100, C424S600000, C424S725000

Reexamination Certificate

active

07048915

ABSTRACT:
The present invention provides a composition for cell-free protein synthesis, which is superior in storage stability in a freeze-dried state, more particularly a freeze-dryable or freeze-dried composition for cell-free protein synthesis, which contains a cell extract for cell-free protein synthesis and inositol, and a freeze-dryable or freeze-dried composition for cell-free protein synthesis containing a cell extract for cell-free protein synthesis, and a deliquescent material in a proportion of not more than 0.01 part by weight per part by weight of a protein in the composition; and a composition for cell-free protein synthesis superior in storage stability in a frozen state, more particularly a freezable or frozen composition for cell-free protein synthesis, containing a cell extract for cell-free protein synthesis and polyhydric alcohol.

REFERENCES:
patent: 6653463 (2003-11-01), Chen et al.
patent: 2003/0162246 (2003-08-01), Endo et al.
patent: WO 98/02532 (1998-01-01), None
Endo et al., “Production of an Enzymatic Active Protein Using a Continuous Flow Cell-Free Translation System,”Journal of Biotechnology, 25, 221-230 (1992).
Erickson et al., “Cell-Free Translation of Messenger RNA in a Wheat Germ System,”Methods in Enzymology, 96, 38-50 (1996).
Madin et al., “A Highly Efficient and Robust Cell-Free Protein Synthesis System Prepared from Wheat Embryos: Plants Apparently Contain a Suicide System Directed at Ribosomes,”Proceedings of the National Academy of Sciences of the United States of America, 97 (2), 559-564 (Jan. 18, 2000).

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