Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1984-12-31
1987-03-31
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435800, 435948, 530380, C12N 500, C12R 191, C07K 1506
Patent
active
046543040
ABSTRACT:
The present inventiona is directed to a mammalian serum-originated growth factor-containing composition useful in cell cultivation. The composition of the present invention is substantially free of active microorganisms and other harmful substances which would otherwise interfere with cell cultivation. Preferably the composition of the present invention is totally free of contaminant microorganisms, i.e., is sterile.
The present invention is also directed to a method of producing the composition of the invention. One step of this method comprises the inactivation of any contaminant microorganisms present in the starting serum. Another step comprises the salting out and desalting of the starting serum to obtain a fraction of the serum which contains the desired cell growth factor. Either step may be conducted first.
Finally the present invention provides a medium for cell cultivation which medium contains the present composition together with a basal medium, as well as a cultivation composition which contains the medium and the cells.
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Kyozo Tsukamoto
Seijiro Sasai
Tadanobu Fujimoto
Conlin David G.
Linek Ernest V.
Maurey Karen
Takeda Chemical Industries Ltd.
Wiseman Thomas G.
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