Composition for assaying for nitrites

Details Composition for assaying for nitrites Composition for assaying for nitrites

1984-12-17

1986-12-23

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

436110, C12Q 112, G01N 3300

Patent

active

046312552

ABSTRACT:
A composition for assaying nitrites comprising a diazotizable amine, an acid component and a coupling component, wherein the coupling component is a compound of the following formula I: ##STR1## wherein R represents a hydrogen atom or a straight-chain alkyl group having 1 to 6 carbon atoms, such as a methyl, ethyl, n-propyl, n-butyl, n-pentyl or n-hexyl group and n represents an integer of 1 to 6, with the proviso that the sulfoalkyl group may be substituted with at least 1 hydroxyl group,
or a water-soluble salt thereof as well as test devices carrying the composition. This composition is far more sensitive than conventional compositions for assaying nitrites. With this composition, for example, a trace amount of as small as 0.02 mg/100 ml of a nitrite to be detected in urine in the diagnosis of a bacterial infection such as urinary tract infection can be detected surely.

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