Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...
Reexamination Certificate
2011-04-19
2011-04-19
Fronda, Christian L (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
C435S252300, C435S320100, C536S023200
Reexamination Certificate
active
07927850
ABSTRACT:
The present invention relates to the isolation and functional identification of a novel acid and heat resistant trehalose synthase enzyme cloned fromPicrophilus torridus. More particularly, the present invention discloses the DNA sequence for thePicrophilus torridustrehalose synthase gene, PTTS, which when expressed in a heterologous host such asEscherichia coli, provides enzymatic activity that catalyzes the direct interconversion of maltose and trehalose through intramolecular transglycosylation. Additionally, the present invention teaches methods of use of PTTS for production of trehalose as well as for production of various useful compounds comprising trehalose.
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Chen Yi-Shan
Lee Guan-Chiun
Shaw Jei-Fu
Academia Sinica
Fronda Christian L
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