Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1993-04-16
1995-06-06
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435212, 4352401, 424 942, 424 9465, 424 9467, C12N 950, C12N 948, C12N 500, A61K 3762
Patent
active
054222613
ABSTRACT:
Proteolytic enzyme compositions and processes for digesting connective tissue are disclosed. The enzyme compositions include collagenase, which is essentially free of toxins and non-collagen specific components, and chymopapain, which is essentially free of toxins. The enzyme compositions are used for dissociating microvessel cells from connective tissue. Recovered microvessel cells are incorporated into artificial vessel grafts. The enzyme compositions preferably contain an aqueous mixture of collagenase having an activity of about 43 nkat/ml to about 51 nkat/ml, and chymopapain having an activity of about 0.22 nkat/ml to about 0.44 nkat/ml.
REFERENCES:
patent: 3558433 (1971-01-01), Stern
patent: 4142999 (1979-03-01), Bloching et al.
patent: 4167447 (1979-09-01), Masri et al.
patent: 4212945 (1980-07-01), Nonaka
patent: 4212946 (1980-07-01), Nonaka
patent: 4374926 (1983-02-01), Stern
patent: 4439423 (1984-03-01), Smith
patent: 4719108 (1988-01-01), Smith
patent: 4812314 (1989-03-01), Barenholz
patent: 4978332 (1990-12-01), Luck
patent: 5116615 (1992-05-01), Gokcen
patent: 5227157 (1993-07-01), McGinity et al.
C. C. Worthington, ed., "enzymes and related biochemicals", Worthington Enzyme Manual, 1988, pp. 93-95.
M. Maeda, "Studies on the characteristics of human lung mast cells . . . " Okayama Igakkai Zasshi, 1986, 98(5-6), pp. 475-486.
I. Marayuma et al., "Preparation of Single Smooth Muscle Cells . . . Collagenase and Papain", J. Pharmacol. Methods, Sep. 1987, 18(2), pp. 151-161.
B. J. Bolzon & D. W. Cheung, "Isolation and Characterization of Vascular Smooth Muscle Cells . . . ", Hypertension, Aug. 1989, pp. 137-144.
I. Maruyama et al., "Improvement of a Procedure . . . Collagenase and Papain", J. Pharmacol. Methods, 1988, 19(2), pp. 155-164.
E. Lammel et al., "Suppression of Steady Membrane Currents by Acetylcholine in Single Smooth Muscle Cells . . . ", J. Physiol., 1991, 432, pp. 259-282.
K. Momose, "Isolation, configuration and contractile responses of single smooth muscle cells", Nippon Yakurigaku Zasshi, Mar. 1993, 101(3), pp. 101-110.
Tavakol, K. et al. "Enhanced dissolution of nucleus pulposus: Combined enzyme approach" Surgical Forum (USA), 1986, vol. 37 pp. 491-494.
Dinh Tan T.
Hornacek Cynthia
Lee Catherine T.
Baxter International Inc.
Condino Debra
Meller Mike
Naff David M.
LandOfFree
Composition containing collagenase and chymopapain for hydrolyzi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Composition containing collagenase and chymopapain for hydrolyzi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Composition containing collagenase and chymopapain for hydrolyzi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-987109