Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1993-03-11
1994-06-07
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435832, 43525231, 162158, C12N 942, C12N 122, C12N 100, D21F 1100
Patent
active
053189051
ABSTRACT:
A cellulase is provided having the following properties: (1) an optimum pH of from 9.5 to 10.5 as measured using carboxymethylcellulose as a substrate; (2) a stable pH of from 6 to 11 as measured using carboxymethylcellulose as a substrate after treating at 30.degree. C. and for 30 minutes; (3) an optimum temperature of about 55.degree. C. as measured using carboxymethylcellulose as a substrate; (4) influence of a surfactant being such that residual activity is 90% or more after treatment at 30.degree. C. and pH of 9.0 for 2 hours in the presence of a sodium n-alkylbenzenesulfonate; (5) a molecular weight of 52,000.+-.2,000 as measured by electrophoresis on SDS-polyacrylamide gel; and (6) an isoelectric point of 4.2.+-.0.2 as measured by isoelectric electrophoresis. The cellulase is produced by Bacillus FERM BP-3431 or a mutant strain thereof. Also disclosed is a detergent comprising the cellulase, a builder and a surfactant. A composition and a method of treating a paper pulp slurry is also disclosed.
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Masatsuji Eiko
Saito Kiyoshi
Seko Masahiko
Meller Michael V.
Naff David M.
Showa Denko K.K.
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