Composition comprising an endosomolytic agent for introducing nu

Details Composition comprising an endosomolytic agent for introducing nu Composition comprising an endosomolytic agent for introducing nu

1995-05-25

1999-11-09

Crouch, Deborah

Chemistry: molecular biology and microbiology

Vector, per se

514 44, 424 932, 4352351, 536 231, A61K 4800, C07H 2102, C12N 700

Patent

active

059812737

ABSTRACT:
A composition for the transfection of higher eucaryotic cells, comprising complexes of nucleic acid, a substance having an affinity for nucleic acid and optionally an internalizing factor, contains an endosomolytic agent, e.g. a virus or virus component, which may be conjugated. The endosomolytic agent, which is optionally part of the nucleic acid complex, is internalized into the cells together with the complex and releases the contents of the endosomes into the cytoplasm, thereby increasing the gene transfer capacity. Pharmaceutical preparations, transfection kits and methods for introducing nucleic acid into higher eucaryotic cells by treating the cells with the composition are also disclosed.

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