Composition comprising a cell expressing an AC133 cell...

Drug – bio-affecting and body treating compositions – Conjugate or complex of monoclonal or polyclonal antibody,...

Reexamination Certificate

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C424S153100, C530S391100

Reexamination Certificate

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08084033

ABSTRACT:
Compositions comprising a cell expressing an AC133 cell surface antigen and an antibody, or antigen binding fragment thereof, that specifically binds to the antigen are provided. Expression of the antigen is highly tissue specific. It is detected on a subset of hematopoietic progenitor and stem cells derived from human bone marrow, fetal bone marrow and liver, cord blood and adult peripheral blood. The subset of cells recognized by AC133 is CD34brightand contains substantially all of the CFU-GM activity present in the CD34+population. This highly specific distribution of AC133 makes it exceptionally useful as a reagent for isolating and characterizing human hematopoietic progenitor and stem cells. Cells selected for expression of AC133 antigen can be further purified by selection for other hematopoietic stem cell and progenitor cell markers.

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