Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose
Patent
1980-09-02
1982-10-12
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving glucose or galactose
23230B, 23901, 252408, 422 56, 435 28, 435805, G01N 3366, G01N 3352
Patent
active
043539840
DESCRIPTION:
BRIEF SUMMARY
DESCRIPTION
1. Field of the Invention
This invention relates to an improvement of the test piece for measuring the concentration of glucose being contained in the body fluid such as urine, spinal fluid, blood, and the like.
2. Description of the Prior Art
The measurement of the concentration of glucose in the body fluid is of importance in the clinical examination, and it is particularly necessary to detect the glucose in urine, and measure its concentration, for the early detection, diagnosis, and control of diabetes and other diseases coexistent therewith.
As a test method to attain such objects, it is preferable to measure rapidly and easily without using any instrument, and furthermore it must have the sufficient exactitude helpful to the diagnosis.
A test piece for glucose has been used heretofore for these purposes. This test piece is made by dipping an absorptive material into a solution containing glucose oxidase, peroxidase, oxidizable chromogen and a buffer agent, and then by drying it. If the thus produced test piece is dipped into the urine, it will be colored corresponding to the concentration of glucose in the urine, upon the principle which is expressed schematically below. The detection and measurement of glucose become practicable through the judgement of this chroma. ##STR1##
As an oxidizable chromogen, there has been used heretofore a chromogen indicator such as orthotolidine, benzidine, orthodianisidine and the like. However, in the case of detecting or measuring glucose in the urine through the application of a test piece made with the use of these indicators, the color reaction will be affected by the specific gravity (the degree of concentration or dilution) of the urine, otherwise by reducing medical substance such as vitamin C (ascorbic acid) coexisting together with the urine, which entail a serious defect incurring big errors in the measurement. Not only that, these kinds of chromogen indicators have carcinogenicity, and therefore encounter a problem for reasons of safety and the sanitation of persons engaged in their production and furthermore their users.
In order to eliminate these defects, there is disclosed in the Japanese Patent Publication No. 39558 of 1975 a test piece for semi-determining the glucose which is coated on its surface with a hydrophobic film-forming polymer such as cellulose ether and ester while applying a water soluble iodine salt as a chromogen. As being understood from the above, this invention uses a, water soluble iodine salt as a chromogen. In order to make this chromogen function effectively as an indicator material, the test piece according to this patent is coated with a hydrophobic film-forming polymer, whereby it claims to be able to prevent the harmful influence from vitamin C (ascorbic acid).
It was proved, however, in the light of the thorough investigation conducted by the author of our invention from an independent standpoint that, as compared with the case where usual chromogen indicators are used, the test piece disclosed in the above-mentioned patent has several defects such that it is 2-3 times longer in the reaction time, that its detection sensitivity is poor to the existence of glucose in the urine (more than 100 mg/dl), and that it is wanting in the stability of long-term conservation due to the lack of stability of water soluble iodine salt impregnated in that test piece.
Through the elimination of the above-mentioned defects, our present invention has for its object the provision of such a kind of test piece for measuring the glucose in the urine quickly and easily, where its measured result is not affected by the composition of the examined body fluid, its detection sensitivity is sufficient for clinical examination, is excellent in the stability of conservation, and can avoid the use of any carcinogenic chromogen.
SUMMARY OF THE INVENTION
Our invention is one where the test piece for measuring the glucose in the body fluid has its substrate impregnated or painted with an enzymatic species displaying glucose oxidase activity,
REFERENCES:
patent: 3092465 (1963-06-01), Adams
patent: 3598704 (1971-08-01), Dahlqvist
patent: 3971702 (1976-07-01), Maekawa
patent: 4066408 (1978-01-01), Jonsson
patent: 4318985 (1982-03-01), Bauer
Yamada Shigeki
Yamamoto Takao
Kabushiki Kaisha Kyoto Daiichi Kagaku
Marantz Sidney
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