Surgery – Truss – Pad
Patent
1985-03-15
1987-08-18
Howell, Kyle L.
Surgery
Truss
Pad
128763, A61B 500
Patent
active
046870008
DESCRIPTION:
BRIEF SUMMARY
The present invention relates to a method of treating a blood sample with an anticoagulant.
In connection with the analysis of whole blood it is known to employ sampling equipment containing anticoagulant in a dry or liquid form for the prevention of coagulation of the sample.
In Hill, A. B. et al., Anesthesiology, September 1979, p. S207, it is disclosed that the use of heparin as anticoagulant in blood samples reduces the potentiometrically determined electrolyte values in the sample, because the heparin is capable of binding a certain amount of the electrolyte.
Radiometer A/S, Copenhagen, has previously developed and marketed liquid anticoagulant compositions for use in connection with blood sampling syringes and sampling equipment in the form of capillary tubes containing a coating on the tube inner wall of a dry anticoagulant composition wherein the anticoagulant composition is especially adapted for use in connection with the sampling of blood which is to be subsequently analysed for the content of ionized calcium. These anticoagulant compositions comprise sodium heparinate, an adjusted amount of calcium chloride and, if desired, water.
The calcium chloride content is adjusted such that the amount of ionized calcium which is bound by the heparinate when brought in contact with the blood sample is provided by the calcium chloride present in the anticoagulant composition. Thereby the resulting content of ionized calcium in the blood sample will be essentially the same as the original content irrespective of the addition of calcium binding heparinate.
The present invention is based on the finding that it is possible to compensate for the anticoagulant binding of several cation species so that the contents of said cation species in one and the same blood sample may be determined by a subsequent analysis without any substantial errors.
Thus, the present invention provides a method for treating a blood sample with an anticoagulant, said method comprising: receptacle, the anticoagulant, which is capable of binding different cation species in the blood, and selected ones of said different cation species in amounts compensating for proportions of the selected cation species bound by the anticoagulant so as to obtain substantially the same concentration of the selected cation species in an unbound condition in the anticoagulant treated blood sample as in the untreated blood sample.
The compensation for the proportions of the selected cation species bound by the anticoagulant is preferably so that the relative error caused by the binding to the anticoagulant is less than or equal to 0.5 percent, in particular less than or equal to 0.35 percent.
The provision of such a universal method of treating a blood sample with an anticoagulant is a significant advance. The user thereby avoids having to work with various treatment methods and/or sampling equipment and/or anticoagulants depending on which subsequent potentiometric analysis is to be carried out, which, among other things, has the advantage of eliminating the otherwise clear risk of mistake. In other words, by employing the method according to the invention it is possible to attain correct analytical results for different components by means of a single blood sample. This is not only of importance from the point of view of saving time and reducing the consumption of materials but also from the point of view of the patient, as the number of blood sample withdrawals is reduced. Moreover, many analytical instruments are adapted to analyse the blood sample with respect to particular combinations of cations or electrolytes, and the present invention makes it possible to provide blood sampling receptacles and anticoagulant compositions dedicated to such combinations.
The amount of anticoagulant used should on the one hand ensure the blood sample against coagulation, but should on the other hand be as small as possible in order to minimize the interference with the blood sample. The selected cation species are preferably selected from the group consisting of hydroge
REFERENCES:
patent: 4252118 (1981-02-01), Richard et al.
patent: 4479799 (1984-10-01), Thiel
patent: 4571382 (1986-02-01), Adachi
Eisenhardt Anne R.
Hansen Ole M.
Citrin Randy
Howell Kyle L.
Radiometer A/S
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