Composition and methods of using the Mirabilis mosaic...

Details Composition and methods of using the Mirabilis mosaic... Composition and methods of using the Mirabilis mosaic...

2005-08-16

2005-08-16

Ketter, James (Department: 1636)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S320100, C435S419000, C536S023100

Reexamination Certificate

active

06930182

ABSTRACT:
The isolation of and methods of using a sub-genomic transcript (Sgt) promoter fromMirabilis mosaicvirus (MMV) are described. A 333 bp MMV Sgt promoter fragment (sequence−306 to +27 from the transcription start site, TSS) was found to be sufficient for strongest promoter activity. This MMV Sgt promoter fragment shows comparable promoter activity to the MMV FLt promoter both in transgenic plants and in protoplasts. The MMV Sgt promoter also demonstrates much greater activity compared toCauliflower mosaicvirus (CaMV) 19S promoter and 35S promoter. The MMV Sgt promoter fragment and any chimeric gene to which it may be linked are usefull for plant geneic engineering to obtain transgenic plants, plant cells and seeds.

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