Composition and method of assaying aqueous liquids for specific

Details Composition and method of assaying aqueous liquids for specific Composition and method of assaying aqueous liquids for specific

1988-07-05

1991-10-08

Warden, Robert J.

Chemistry: analytical and immunological testing

Process or composition for determination of physical state...

422 57, 436169, G01N 3352

Patent

active

050554077

ABSTRACT:
A new and improved composition and method of determining the specific gravity of an aqueous test sample. The method includes using a test device comprising a carrier matrix incorporating a reagent composition capable of interacting with an aqueous test sample to produce a visually or instrumentally detectable and measurable response that correlates to the specific gravity of the aqueous test sample. The new and improved reagent composition, comprising a molybdate-dye complex indicator, such as a molybdatepyrocatechol violet complex; a protein, such as albumin; a chelating agent, like tartaric acid; and, if necessary, a suitable buffer, is incorporated into a carrier matrix, like filter paper, to provide increased sensitivity to test sample specific gravity and improved color differentiation between test samples having different specific gravities, thereby affording a more accurate and trustworthy specific gravity assay of an aqueous test sample, such as urine.

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Fujita et al., "Color Reaction Between Pyrogallol Red-Molybdenum (IV) Complex and Protein", Bunsekikagaku vol. 32, pp E379-E387, 1983.
Watanabe et al., "Urinary Protein as Measured with a Pyrogallol Red-Molybdate Complex, Manually and in a Hatachi 726 Automated Analyzer", Clin. Chem. 32/8, pp. 1551-1554 (1986).

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