Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol
Patent
1977-10-13
1980-01-01
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving cholesterol
G01N 3114
Patent
active
041815750
ABSTRACT:
In a process for the determination of total or bound cholesterol by liberating any bound cholesterol using cholesterol esterase and simultaneously or subsequently determining the liberated cholesterol and any initially free cholesterol with a cholesterol reacting enzyme, the improvement comprising using as the cholesterol reacting enzyme an NAD or NADP-dependent cholesterol dehydrogenase obtained from an anaerobic microorganism, e.g., Eubacterium sp. ATCC 21408, or from mammalian tissue, e.g., liver.
REFERENCES:
patent: 3907642 (1975-09-01), Richmond
Marcus, et al., "Induction and Purification of .alpha.- and .beta.-Hydroxy Steroid Dehydrogennses", J. Biol. Chem. vol. 218, (1956) pp. 661-673.
Talalay, et al., "Specificity, Kinetics, and Inhibition of .alpha.- and .beta.-Hydroxysteroid Dehydrogennses", J. Biol. Chem., vol. 218 (1956) pp. 675-690.
Parmentier et al., "Mechanism of Brohydrogenation of Cholesterol to Coprostanol by Eubacterium ATCC 21408", Biochem. Biophys. Acta, vol. 348 (1974) pp. 279-284.
Curish, "Cholesterol Oxidase, A Literature Review", Beckman Instruments, Inc. 1969.
Klingenberg, Methods of Enzymatic Analysis by H. U. Bergmeyer, 1974 pp. 2045-2059.
Gruber Wolfgang
Lang Gunter
Nelboeck-Hochstetter Michael
Roeschlau Peter
Seidel Hans
Boehringer Mannheim GmbH
Wiseman Thomas G.
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