Composition and method for the determination of ascorbic acid

Chemistry: physical processes – Physical processes – Crystallization

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23230B, 23904, 1951035R, 252408, 422 57, G01N 2106, G01N 3114, G01N 3316

Patent

active

041521161

ABSTRACT:
Ascorbic acid or dehydroascorbic acid is determined by reaction with a tetrazolium salt selected from 3-(4,5-dimethyl-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide, nitrotetrazolium blue, tetrazolium blue chloride and distyryl-nitro blue tetrazolium chloride, in the presence of phenazine methosulphate and a detergent, at a weakly acidic pH value, and evaluating the coloration obtained as a measure of the initial acid content. The method is particularly efficacious in substantially eliminating disturbance of the reaction by foreign substances.

REFERENCES:
patent: 3771964 (1973-11-01), Fader
patent: 4056485 (1977-11-01), Adolf
Chemical Abstracts, 77: 137049g (1972).
Chemical Abstracts, 82: 70120f (1975).

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