Composition and method for the detection of hydrogen peroxide

Chemistry: analytical and immunological testing – Oxygen containing – Ozone or peroxide

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422 56, 422 61, 435 28, 436904, 548358, G01N 2178, G01N 3350

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active

044395272

ABSTRACT:
Means and method for the detection of hydrogen peroxide or of hydrogen peroxide-forming substrates, consisting of 4-aminoantipyrine and an aromatic amine or phenol and a neutral to weakly acid buffer as well as other additives and reagents which form H.sub.2 O.sub.2 with the substrate and which are known for use in Trinder's reaction, the 4-aminoantipyrine, in the immiscible state before use, being separate from the acid buffer and from the other reagents if any, and being mixed with a small amount of an alkaline buffer having a pH of 8.5 to 14.0, the two compositions being combined, in a quantity ratio to one another that is suitable for the detection test, in a single package or in a single-use unit.

REFERENCES:
patent: Re29498 (1977-12-01), Meiattini
patent: 4078892 (1978-03-01), Steinbrink, Jr.
patent: 4098574 (1978-04-01), Dappen
patent: 4247631 (1981-01-01), Nix et al.
patent: 4251629 (1981-02-01), Yamanisi et al.
Grillo et al., "Clinical Chemistry", 27/3, 375-379 (1981).

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