Composition and method for targeted integration into cells

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

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4353201, 435468, 435473, C12N 1564, C12N 1581, C12N 1582, C12N 1585

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active

059587750

ABSTRACT:
A vector containing a selected nucleic acid sequence interposed between the terminal ends of a Tn7 transposon and a method for site specific integration of genetic material into a chromosome using those vectors are provided.

REFERENCES:
patent: 4590162 (1986-05-01), Grinter et al.
patent: 4784956 (1988-11-01), Grinter et al.
patent: 5102797 (1992-04-01), Tucker et al.
Rogers et al., Mol. Gen. Genet. 205:550-556 (1986).
Alton et al., "Non-invasive liposome-mediated gene delivery can correect the ion transprot defect in cystic firbrosis mutant mice", Nature Genet. 1993, 5, 135-142.
Arciszewska et al., Transposon Tn7 cis-Acting Sequences in Transposition and Transposition Immunity, J. Mol. Biol. 1989, 207, 35-52.
Arciszewska et al., Purification of TnsB, a Transposition Protein That Binds to the Ends of Tn7, J. Biol. Chem. 1991, 266, 21736-21744.
Arciszewska, L. and Craig, N.,"Interaction of the Tn7-encloded transposition protein TnsB witht he ends of the transposon", Nuc. Acids Res. 1991, 19, 5021-5029.
Bainton et al., "Tn7 Transposition: Target DNA Recognition Is Mediated by multiple Tn7-Encoded Proteins in a Purified In Vitro System", Cell 1993, 72, 931-943.
Chalfie et al., "Green Fluorsecent Protein as a marker for Gene Expression", Science 1994, 263, 802-805.
Chu et al., "Electroporation for the efficient transfection of mammalian cells with DNA", Nucleic Acids Res. 1987, 1311-1326.
Craig, N.L., Transposon Tn7, Curr. Topics Micorbiol. Immunol. 1996, 204 27-48.
Gamas, P. and Craig, N.L., "Purification and characterizationo f TnsC, a Tn7 transposition protein that binds ATP and DNA", Nuc. Acids Res. 1992, 20, 2525-2532.
Gargia-Buston et al., "Nuclear protein localization", Biochim. Biophys. Acta 1991, 1071, 83-101.
Gary et al., "Multiple DNA Processing Reactions Underlie Tn7 Transposition", J. Mol. Biol. 1996, 257, 301-316.
Hayward et al., "Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis", Nature 1981 290, 475-480.
Hyde et al., "Correction of the ion transport defect in cycstic fibrosis transgenic mice by gene therapy", Nature 1993, 362, 250-255.
Kotin et al., Site-specific integration by adeno-associated virus, Proc. Natl. Acad. Sci. USA 1990, 87, 2211-2215.
Kubo, K. and Craig, N., "Bacterial Transposon Tn7 Utilizes Two Different Classes of Target Sites", J. Bacteriol. 1990, 172, 2774-2778.
Lichtenstein, C. and Brenner, S., "Unique insertion site of Tn7 in the E. Coli chromosome", Nature 1982, 297, 601-603.
McKnight et al., "Molecular Cloning, cDNA Sequence, and Bacterial Expression of Human Glutamine:Fructose-6-phosphate Amidotransferase", J. Biol. Chem. 1992, 267, 25208-25212.
McKown et al., "Sequence Requirements of Es herichia coli attTn7, a Specific Site of Transposon Tn7 Insertion", J. Bacteriol. 1988, 170, 352-358.
Orle, K. Craig, N., "Identification of transposition proteins encoded by the bacterial transposon Tn7", Gene 1991, 104, 125-131.
Richards, T.C. and Greengard, O., "Distribution of Glutamine hexosephosphate Aminotransferase in Rat Tissues; Changes with State of Differentiation", Biochim. Biophys. Acta 1973, 304, 842-850.
Rothstein, R. Methods of Enzymology, vol. 194, Guide to Yeast Genetics and Molecular Biology, Academic Press, (1991) CHap. 19, 281-301.
Sayeski et al., "The murine glutamine:fructose-6-phosphate amidotransferase-encoding cDNA sequence", Gene 1994, 140, 289-190.
Waddel, C. and Craig, N.,"Tn7 transposition: Recognition of the attTn7 target sequenece", Proc. Natl. Acad. Sci. USA 1989, 86, 3958-3962.

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