Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1984-01-16
1987-09-15
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 19, 435 29, 435 39, 435269, 2504612, C12Q 104, C12Q 144, C12Q 102, C12Q 106, G07K 300, G01N 2164
Patent
active
046939725
ABSTRACT:
A method for rapid detection of microorganisms in a body fluid sample includes detecting the microorganisms after treatment of the sample with a lysing agent in order to dissolve sample components other than microorganisms, and staining with a fluorescent dye. The lysing agent may be part of a composition which includes a culture medium thereby providing simultaneous lysis and growth before staining. Detection is preferably accomplished by reliance on the fluorescence emitted by the dye having been properly excited by light energy.
A composition suitable for use in the above-described method includes a growth medium and a lysing agent.
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Mansour James D.
Neece Vernon R.
Schulte Thomas H.
Becton Dickinson and Company
Brown Richard E.
Foley Shawn P.
Kepplinger Esther M.
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