Chemistry: natural resins or derivatives; peptides or proteins; – Natural resins or derivatives
Patent
1997-03-28
2000-07-04
Lankford, Jr., Leon B.
Chemistry: natural resins or derivatives; peptides or proteins;
Natural resins or derivatives
435366, 435325, 514 2, C12N 500
Patent
active
060840609
ABSTRACT:
The invention relates to a protein material which is effective to preserve progenitor cells, such as hematopoietic progenitor cells. The protein has an amino acid sequence comprising AQSLSFSFTKFD (SEQ ID NO:1) and a molecular weight of about 12-20 kD, or has an amino acid sequence comprising VVAVEFD (SEQ ID NO:3) and a molecular weight of about 15-20 kD. Heterodimers of the protein are described, and multimers thereof. Methods of using the protein of the invention for preserving progenitor cells in vitro, ex vivo, and in vivo are also described. The invention, therefore, include methods such as myeloablation therapies for cancer treatment wherein myeloid reconstitution is facilitated by means of the specified protein. Other therapeutic utilities are also enabled through the invention, for example, expanding progenitor cell populations ex vivo to increase chances of engraftation, improving conditions for transporting and storing progenitor cells, and facilitated gene therapy to treat and cure a broad range of life-threatening hematologic diseases.
REFERENCES:
patent: 4808611 (1989-02-01), Cosman
patent: 5186931 (1993-02-01), Kishimoto et al.
patent: 5472867 (1995-12-01), Kanz et al.
Altabella T and Chrispeels MJ, "Tobacco Plants Transformed with the Bean .alpha.ai Gene Express an Inhibitor of Insect .alpha.-Amylase in Their Seeds", Plant Physiol. 93:805-810 (1990).
An G, Ebert PR, Mitra A and Ha SB, "Binary Vectors", Plant Molecular Biology Manual A3:1-19 (1988).
Barondes SH, "Bifunctional Properties of Lectins: Lectins Redefined", Trends in Biochemical Sciences 13:480-482 (1988).
Berardi AC, Wang A, Levine JD, Lopez P and Scadden DT, "Functional Isolation and Characterization of Human Hematopoietic Stem Cells", Science 267:104-108 (1995).
Borge OJ, Ramsfjell V, Veiby OP, Murphy Jr. MJ, Lok S and Jacobsen SEW, "Thrombopoietin, But Not Erythropoietin Promotes Viability and Inhibits Apoptosis of Multipotent Murine Hematopoietic Progenitor Cells In Vitro", Blood 88(8):2859-2870 (1996).
Dexter and Spooncer, "The Structure of the Hemopoietic System", Cell Biol. 3:423-441 (1987).
Dosil M, Wang S and Lemischka IR, "Mitogenic Signalling and Substrate Specificity of the Flk2/Flt3 Receptor Tyrosine Kinase in Fibroblasts and Interleukin 3-Dependent Hematopoietic Cells", Molecular and Cellular Biology 13(10):6572-6585 (1993).
Gabius H-J, "Non-Carbohydrate Binding Partners/Domains of Animal Lectins", Int. J. Biochem 26(4):469-477 (1994).
Hao Q-L, Thiemann FT, Petersen D, Smogorzewska EM and Crooks GM, "Extended Long-Term Culture Reveals a Highly Quiescent and Primitive Human Hematopoietic Progenitor Population", Blood 88(9):3306-3313 (1996).
Higgins TJV, Newbigin EJ, Spencer D, Llewellyn DJ and Craig S, "The Sequence of a Pea Vicilin Gene and its Expression in Transgenic Tobacco Plants", Plant Molecular Biology 11:683-695 (1988).
Hoffman LM, Ma Y, Barker RF, "Molecular Cloning of Phaseolus Vulgaris Lectin mRNA and Use of cDNA as a Probe to Estimate Lectin Transcript Levels in Various Tissues" Nucleic Acids Research 10(23):7820-7829 (1982).
Kuriyama M, Harada N, Kuroda S, Yamamoto T, Nakafuku M, Iwamatsu A, Yamamoto D, Prasad R, Croce C, Canaani E and Kaibuchi, K, "Identification of AF-6 and Canoe as Putative Targets for Ras*", the Journal of Biological Chemistry 271(2):607-610 (1996).
Marfatia SM, Lu RA, Branton D and Chishti AH, "Identification of the Protein 4.1 Binding Interface on Glycophorin C and p55, a Hmologue of the Drosophila discs-large Tumor Suppressor Protein*", The Journal of Biological Chemistry 270(2):715-719 (1995).
Moreno J and Chrispeels MJ, "A Lectin Gene Encodes the .alpha.-amylase Inhibitor of the Common Bean", Proc. Natl. Acad. Sci. USA 86:7885-7889 (1989).
Mosmann T, "Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays", Journal of Immunological Methods 65:55-63 (1983).
Opdenakker C, Rudd PM, Wormald M, Dwek RA and Van Damme J, "Cells Regulate the Activities of Cytokines by Glycosylation", The FASEB Journal 9:453-457 (1995).
Pueyo JJ, Chrispeels MJ and Herman EM, "Degradation of Transport-Competent Destabilized Phaseolin with a Signal for Retention in the Endoplasmic Reticulum Occurs in the Vacuole", Planta 196:586-596 (1995).
Shah AJ, Smogorzewska EM, Hannum C and Crooks GM, "Flt3 Ligand Induces Proliferation of Quiescent Human Bone Marrow CD34.sup.+ CD38.sup.- Cells and Maintains Progenitor Cells In Vitro", Blood 87(9):3563-3570 (1996).
Small D, Levenstein M, Kim E, Carow C, Amin S, Rockwell P, Witte L, Burrow C, Ratajczak MZ, Gewirtz AM and Civin CI, "STK-1, the Human Hmolog of Flk-2/Flt-3, is Selectively Expressed in CD34.sup.+ Human Bone Marrow Cells and is Involved in the Proliferation of Early Progenitor/Stem Cells", Proc. Natl. Acad. Sci. USA 91:459-463 (1994).
Tessler S, Rockwell P, Hicklin D, Cohen T, Levi B-Z, Witte L, Lemischka IR, Neufeld G, "Heparin Modulates the Interaction of VEGF.sub.165 with Soluble and Cell Associated flk-1 Receptors", The Journal of Biological Chemistry 269(17):12456-12461 (1994).
Turhan AG, Humphries K, Phillips GL, Eaves AC and Eaves CJ, "Clonal Hematopoiesis Demonstrated by X-Linked DNA Polymorphisms After Allogeneic Bone Marrow Transplantation", The New England Journal of Medicine 320(25):1655-1661 (1989).
Young JC, Varma A, DiGiusto D and Backer MP, "Retention of Quiescent Hematopoietic Cells with High Proliferative Potential During Ex Vivo Stem Cell Culture", Blood 87(2):545-556 (1996).
Zipori D, "Regulation of Hemopoiesis by Cytokines that Restrict Options for Growth and Differentiation", Cancer Cells 2(7):205-211 (1990).
Lalitha R. Gowda, Handanahal S. Savithri, and D. Rajagopal Rao, "The Complete Primary Structure of a Unique Mannose/Glucose-specific Lectin from Field Bean (Dolichos labgl lab)," (1994) the Journal of Biological Chemistry 269, 18789-18793.
Nathan Sharon and Halina Lis, "Lectins as Cell Recognition Molecules," (1989) Science 246, 227-234.
Raymond A. Dwek, "Glycobiology: More Functions for Oligosaccharides," (1995) Science 269, 1234-1235.
Dov Zipori, "The Renewal and Differentiation of Hemopoietic Stem Cells," (1992) The FASEB Journal 6, 2691-2697.
Feit Irving N.
ImClone Systems Incorporated
Lankford , Jr. Leon B.
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