Composition and method for isolation and purification of Immunog

Details Composition and method for isolation and purification of Immunog Composition and method for isolation and purification of Immunog

1990-09-28

1992-05-12

Russel, Jeffrey E.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Blood proteins or globulins, e.g., proteoglycans, platelet...

530402, 530411, 530413, 530813, 5303881, 530863, 530864, C07K 320, C07K 1710

Patent

active

051129523

ABSTRACT:
Preparation of a composite of mannan binding protein attached to an insoluble, support matrix is accomplished by reacting cyanogen bromide activated beaded agarose with a buffered solution of mannan binding protein isolated from rabbit serum. The composite has utility as an affinity sorbent for IgM when divalent metal ions are incorporated in the binding buffer. The composite does not show cross-reactivity (binding) with immunoglobulins of the G class.

REFERENCES:
Kozutsimi et al., "Isolation And Characterization Of A Mannan-Binding Protein From Rabbit Serum," Biochem. Biophys. Res. Comm., vol. 95, No. 2, Jul. 31, 1980, pp. 658-664.
Lu et al., "Binding of the Pentamer/Hexamer Forms Of Mannan-Binding Protein . . . ", J. Immunol., vol. 144, No. 6, Mar. 15, 1990, pp. 2287-2294.
Nethery et al, "Single-step purification of immunoglobulin M on Clq-Sepharose," J. Immunol. Methods, vol. 126, 1990, pp. 57-60.
Ohto et al., "The Mechanism of Carbohydrate-mediated Complement Activation by the Serum Mannan-binding Protein," J. Biol. Chem., vol. 265, No. 4, Feb. 5, 1990, pp. 1980-1984.
Shibuya et al., "One-Step Purification of Murine IgM . . . ", Arch. Biochem. Biophys., vol. 267, No. 2, Dec. 1988, pp. 676-680.

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