Composition and method for increasing cell density in cell...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...

Reexamination Certificate

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C424S184100, C424S208100

Reexamination Certificate

active

10140172

ABSTRACT:
A composition and method for enhancing cell growth and increasing the density of cell cultures containing lentivirus-infected host cells comprises adding a suitable quantity of an antibiotic to the culture to destroy harmful bacteria.

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Navenot, J., et al., Molecular Anatomy of CCR5 Engagement by Physiologic and Viral Chemokines and HIV-1 Envelope Glycoproteins, J. Mol. Biol. 2001, vol. 313, pp. 1181-1193.
Yamamoto, J.K., et al., “Development of IL-2-Independent Feline Lymphoid Cell Lines Chronically Infected With Feline Immunodeficiency Virus: Importance for Diagnostic Reagents and Vaccines,”Intervirology,vol. 32, No. 6, pp. 361-375, (1991).
Zapp, M. L., et al., “Small Molecules That Selectivity Block RNA Binding of HIV-1 Rev Protein Inhibit Rev Function and Viral Production,” Cell, vol. 74, No. 6, pp. 969-978, Sep. 24, 1993.
Navenot, J. et al., “Molecular Anatomy of CCR5 Engagement by Physiologic and Viral Chemokines and HIV-1 Envelope Glycoproteins: Differences in Primary Structural Requirements for RANTES, MIP-1a, and vMIP-II Binding,”J. Mol. Biol.,(2001), 313(5):1181-93.

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