Chemistry: analytical and immunological testing – Hemoglobin – myoglobin – or occult blood
Patent
1998-03-24
1999-03-16
Wallenhorst, Maureen M.
Chemistry: analytical and immunological testing
Hemoglobin, myoglobin, or occult blood
436 8, 436 10, 436 15, 436 17, 436 18, 436 63, 436166, 436175, 435 2, 2524081, 424 9371, 424 9373, 424529, 424533, 424534, G01N 3372
Patent
active
058829342
ABSTRACT:
A cyanide-free lytic reagent composition and method for measuring the total hemoglobin concentration in a blood sample, for counting the number of leukocytes and for deferential counting of leukocyte subpopulations are described. The cyanide-free lytic reagent composition cotains a quaternary ammonium salt or a pyridinium salt to lyse erythrocytes and release hemoglobin, and an organic ligand including triazole and its derivatives, tetrazole and its derivatives, alkaline metal salts of oxonic acid, melamine, aniline-2-sulfonic acid, quinaldic acid, 2-amino-1,3,4-thiadiazole, triazine and its derivatives, urazole, DL-pipecolinic acid, isonicotinamide, anthranilonitrile, 6-aza-2-thiothymine, 3-(2-thienyl)acrylic acid, benzoic acid and alkali metal and ammonium salts of benzoic acid, and pyrazine and its derivatives to form a stable chromogen with hemoglobin, and a salt to adjust conductivity of the reagent for impedance measurement. The reagent composition is mixed with a blood sample without pre-dilution and the UV absorption of the sample mixture is measured at the predetermined absorption wavelength. Counting the number of leukocytes and differential counting of leukocyte subpopulations are accomplished simultaneously on an automated cell counter utilizing DC impedance measurement.
REFERENCES:
patent: 2656508 (1953-10-01), Coulter
patent: 4185964 (1980-01-01), Lancaster
patent: 4286963 (1981-09-01), Ledis et al.
patent: 4485175 (1984-11-01), Ledis et al.
patent: 4528274 (1985-07-01), Carter et al.
patent: 4529705 (1985-07-01), Larsen
patent: 4853338 (1989-08-01), Benezra et al.
patent: 5242832 (1993-09-01), Sakata
patent: 5250437 (1993-10-01), Toda
patent: 5612223 (1997-03-01), Kim et al.
patent: 5686308 (1997-11-01), Li et al.
patent: 5763280 (1998-06-01), Li et al.
Oshiro et al. (1982) New method for hemoglobin determination by using sodium lauryl sulfate (SLS). Clin. Biochem. 15(1) 83-88.
Li Yi
Young Carole
Alter Mitchell E.
Coulter International Corp.
Wallenhorst Maureen M.
LandOfFree
Composition and method for hemoglobin and cell analysis does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Composition and method for hemoglobin and cell analysis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Composition and method for hemoglobin and cell analysis will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-816817