Composition and method for enhancing detection of superoxide ani

Details Composition and method for enhancing detection of superoxide ani Composition and method for enhancing detection of superoxide ani

1995-03-24

1996-02-20

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 4, 435 29, 422 52, 436172, 424 944, 424646, C12Q 128, G01N 2176

Patent

active

054928168

ABSTRACT:
A composition and method for enhancing luminol chemiluminescence assays for superoxide anion comprising an orthovanadate salt, such as sodium orthovanadate. Orthovanadate enhances photon emission when luminol is cleaved by superoxide anion. The orthovanadate salt is non-cytotoxic and non-denaturing to intact cells. A method comprises reacting luminol in the presence of an orthovanadate anion with an analyte suspected of containing superoxide anion and measuring the photon output. A kit comprises a predetermined mount of luminol and orthovanadate.

REFERENCES:
patent: 4882171 (1989-11-01), Posner
patent: 5069913 (1991-12-01), Posner
Pilipenko et al, "New Chemiluminescent reactions of luminol in the presence of vanadium salts", Ukv. Khim. zh. (Russ. ed), 39(1), pp. 73-75, 1973.
Yoshino et al, Arch. Biochem. Biophys, vol. 272, No. 1, pp. 76-80, 1989.

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