Composition and method for determining transferase and protease

Details Composition and method for determining transferase and protease Composition and method for determining transferase and protease

1977-08-05

1979-09-11

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 24, G01N 3114, G01N 3316

Patent

active

041674499

ABSTRACT:
Transferase and protease activity in homogenates and biological solutions is fluorometrically determined at wavelengths corresponding generally to those used for fluorometric NADH-linked determinations by utilizing novel substrate compositions consisting essentially of certain fluorogenic 5-aminoisophthalic acid derivatives coupled to amino acid constituents specific to the transferases and proteases under investigation.

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patent: 3862011 (1975-01-01), Smith
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patent: 3892631 (1975-07-01), Carroll
patent: 3979447 (1976-09-01), Bernt et al.
patent: 4070245 (1978-01-01), Svendsen
Wildes et al, "Differences Between Excited States Produced Chemically and Photochemically. Ion Pairs of Excited States Derived From Luminol", J. Amer. Chem. Soc., vol. 95, No. 8, (1973) pp. 2610-2617.
Bayley et al, "Coformational Properties of Pig-heart Cytoplasmic Aspartate Aminotransferase. Circular-Dichromism and Absorption-Steptroscopy Study of Dicarboxylic Binding", Chem. Abstracts. vol. 83, No. 15, p. 223, (1975) Abs. No. 128,197a.

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