Composition and method for determination of cholesterol

Details Composition and method for determination of cholesterol Composition and method for determination of cholesterol

1973-03-01

1980-01-29

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

435190, 435863, 435872, G01N 3114

Patent

active

041862511

ABSTRACT:
The present invention relates to compositions and methods for use in determining free and total cholesterol levels in fluids such as body fluids. A test for free cholesterol is disclosed based on the determination of hydrogen peroxide released through the action of a chemical system having cholesterol oxidase activity on free cholesterol. A preferred means for determining the released hydrogen peroxide involves the use of a substance having peroxidative activity and an oxidation-reduction indicator. The addition of a chemical system having cholesterol ester hydrolase activity to the free cholesterol test composition provides an integral test composition for the determination of total cholesterol.

REFERENCES:
patent: 3092465 (1963-06-01), Adams et al.
patent: 3099605 (1963-07-01), Free
patent: 3183173 (1965-05-01), Oakes
patent: 3607093 (1971-09-01), Stone
patent: 3776816 (1973-12-01), Terada et al.
Clayton, Methods in Enzymology, vol. XV, pp. 528-529, (1969).

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