Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1982-11-08
1985-11-19
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 34, 435 4, 435 6, 435 39, 435243, 435 3, 435184, 435 68, 435245, 435885, 436522, 424 88, 424 92, G01N 3354, C12Q 300, C12Q 100, C12Q 104, C12Q 106, C12N 999, C12N 136, A61K 3902
Patent
active
045542481
ABSTRACT:
A composition is used as a reagent in a method for measuring Antistreptolysin O (ASO) in a blood sample. The composition contains Streptolysin O (SO) at a pH outside the range of its hemolytic activity, e.g., outside the pH range of 5-9. At that pH the SO is reversibly inactivated while maintaining its hemolytic capacity. The composition is employed to measure ASO by mixing one or more samples of non-hemolyzed blood or serum with one or more known quantities of the composition with a known hemolytic capacity, incubating each mixture to allow reaction of any ASO in the sample with the SO, restoring the hemolytic activity of the SO by adjustment of pH to permit lysis and detecting the presence or absence of lysis in each sample.
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Morrison & Boyd, Organic Chemistry, 1977, p. 33, Allyn & Bacon Inc.
Dassy, B. et al., Journal of Gen. Microbiol., vol. 129, pp. 643-651, (1983), "Growth of Streptococcus pyogenes and Streptolysin O Production in Complex and Synthetic Media.
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Green Lorrence H.
Roberts John W.
American Home Products Corporation
Mandel Adley F.
Teskin Robin
Wiseman Thomas G.
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