Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of culturing cells in suspension
Reexamination Certificate
2005-12-06
2005-12-06
Afremova, Vera (Department: 1651)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of culturing cells in suspension
C435S384000, C435S390000, C435S391000, C435S404000, C435S375000, C435S377000
Reexamination Certificate
active
06972195
ABSTRACT:
Compositions and methods are provided for culturing in vitro potentially regenerative cells (PRCs) from which functional tissue-organs are regenerated. In one aspect of the invention, a tissue culture medium is provided which comprises at least 50% of water and a sterol compound that is dissolved in a fatty acid-containing oil at a concentration at least 0.1% by weight based on the weight of the oil and added to the water. The culture medium can be used to culture PRCs that are isolated from the body of a mammal to generate functional tissue-organs in vitro with substantially the same physiological structure and function as the corresponding ones existing in vivo and in situ. The cultured PRCs, tissues, and tissue-organs can serve as valuable models for scientific investigation in life sciences, nutraceutical discovery, drug screening, pharmacokinetic studies, medical devices and tissue/organ transplantation.
REFERENCES:
patent: 5372943 (1994-12-01), Inlow et al.
patent: 5486510 (1996-01-01), Bouic et al.
patent: 5552148 (1996-09-01), Znaiden et al.
patent: 5853755 (1998-12-01), Foldvari
patent: 6833271 (2004-12-01), Bertheussen
Awad et al., FASEB Journal. (Mar. 7, 2001), vol. 15, No. 4, p. A599.
Hoffman et al. Blood. (1981), vol. 57, No. 1, pp. 164-169.
Bhadra et al. Incorporation of liposomal phytosterols into human cells in culture. Biochemical Medecine and Metabolic Biology. 1991. 46, pp. 119-124.
Afremova Vera
Chen Shirley
Rongxiang Xu
Wilson Sonsini Goodrich & Rosati
LandOfFree
Composition and method for culturing potentially... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Composition and method for culturing potentially..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Composition and method for culturing potentially... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3491060