Composition, analytical element and method for the quantificatio

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

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435 15, 435 25, 435805, 435810, 435 28, 435194, 422 56, 422 57, C12Q 129, C12Q 150, C12Q 148, C12Q 126, C12Q 128, C12N 912, G01N 148, G01N 2106

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045474613

ABSTRACT:
An enzymatic method for the analytical determination of creatine kinase in an aqueous liquid such as blood serum is described. The determination is made by measuring an optical density change using the reagents creatine phosphate, adenosine diphosphate, glycerol, glycerol kinase, .alpha.-glycerophosphate oxidase, a chromagen and a mercapto-containing creatine kinase activator. The mercapto-containing creatine kinase activator is added in encapsulated form or in low concentrations so as to preserve the activity of the chromagen.

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Tietz (Ed.), Fundamentals of Clinical Chemistry, W. B. Saunders Co., 1970, pp. 466-470.
Scad. J. Clin. Lab. Invest. 36 (1976), pp. 711-723; Ibid. 39 (1979), pp. 1-5.
Clin. Chem. 27(3), pp. 402-404 (1981).

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