Prosthesis (i.e. – artificial body members) – parts thereof – or ai – Implantable prosthesis – Hair or skin
Patent
1996-02-16
2000-03-21
Isabella, David J.
Prosthesis (i.e., artificial body members), parts thereof, or ai
Implantable prosthesis
Hair or skin
435240, 424424, A61F 210
Patent
active
060397608
ABSTRACT:
Skin equivalents and processes for preparing them are described. The living skin equivalent comprises a layer of cultured keratinocyte cells, a layer of high purity, non-porous collagen and a dermal layer of cultured fibroblast cells in a porous, cross-linked collagen sponge. Processes are described for producing the skin equivalent with descriptions on how to obtain and treat skin precursor materials to yield suitable fibroblasts and keratinocytes.
The collagen layers, the physical forms thereof, and treatments thereof are also described. Preferably, the non-porous, highly-purified collagen is selected from Type 1, Type 3, or mixtures of Type 1 and Type 3 collagen. The collagen is purified ideally by treatment with pepsin, to remove antigenic substances.
The collagen sponge used can be any suitable form of collagen sponge which will support fibroblast growth.
The keratinocytes used in the invention are preferably prepared by the "drop" method spotted evenly on culture media and incubated to coalescence.
REFERENCES:
patent: 4673649 (1987-06-01), Boyce, et al.
patent: 4703108 (1987-10-01), Silver et al.
patent: 4940666 (1990-07-01), Boyce et al.
patent: 5282859 (1994-02-01), Eisenberg
Boyce, et al, Biologic Attachment, Growth, and Differentiation of Cultured Human Epidermal Keratinocytes on a Graftable Collagen and Chondroitin-6-Sulfate Substrate, Surgery, Apr. 1988, pp. 421-430.
Boyce, et al. Biological Attachment, Growth & Differentiation of Cultured Human Epidermal Keratinocytes on Graftable Collagen and Chondroitin-6-Sulfate Substrate; Surgery Apr. 1988, pp. 421-431.
Isabella David J.
Lilore Ralph T.
Ortec International, Inc.
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