Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2005-10-21
2010-06-29
Lu, Frank W (Department: 1634)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S006120, C435S091100, C435S287100, C435S287200, C536S023100, C536S024300, C536S024330
Reexamination Certificate
active
07745178
ABSTRACT:
The presently claimed invention provides for novel methods and kits for reducing the complexity of a nucleic acid sample by providing non-gel based methods for amplification of a subset of the sequences in a sample. In a preferred embodiment, amplification of a subset can be accomplished by digesting a sample with two or more restriction enzymes and ligating adaptors to the fragments so that only a subset of the fragments can be amplified. The invention further provides for analysis of the above amplified sample by hybridization to an array, which may be specifically designed to interrogate the desired fragments for particular characteristics, such as, for example, the presence or absence of a polymorphism.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4800159 (1989-01-01), Mullis et al.
patent: 5093245 (1992-03-01), Keith et al.
patent: 5366877 (1994-11-01), Keith
patent: 5508178 (1996-04-01), Rose et al.
patent: 5807522 (1998-09-01), Brown et al.
patent: 5858656 (1999-01-01), Deugau et al.
patent: 5888737 (1999-03-01), DuBridge et al.
patent: 6004783 (1999-12-01), Ausubel et al.
patent: 6045994 (2000-04-01), Zabeau et al.
patent: 6100030 (2000-08-01), McCasky Feazel et al.
patent: 6124090 (2000-09-01), Rose et al.
patent: 6156502 (2000-12-01), Beattie
patent: 6197557 (2001-03-01), Makarov et al.
patent: 6232067 (2001-05-01), Hunkapiller et al.
patent: 6287825 (2001-09-01), Weissman et al.
patent: 6472185 (2002-10-01), McCasky Feazel et al.
patent: 6514768 (2003-02-01), Guire et al.
patent: 6632611 (2003-10-01), Su et al.
patent: 2003/0113737 (2003-06-01), Pedersen
patent: 534858 (1992-09-01), None
patent: WO 00/18960 (2000-04-01), None
patent: WO 01/75163 (2001-10-01), None
patent: WO 02/20844 (2002-03-01), None
“Frequencies of Restriction Sites” from the online catalog of New England Biolabs, Inc. (www.neb.com).
Lipshutz, et al., BioTechniques 1993):442-47, 1995.
Smith, PCR Methods and Applications 2:21-27, 1992.
Smith, D.R., “Ligation-mediated PCR of Restriction Fragments from Large DNA Molecules”, PCR Methods and Applications, 1992, vol. 2, No. 1, pp. 21-27.
Swa I and Pme I product data sheets, from the online catalog of New England Biolabs, Inc. (www.neb.com).
Vos et al., “EFLP: A New technique for DNA Fingerprinting,” Nucleic Acids Research, 1995, vol. 23 pp. 4407-4414.
Affymetrix Inc.
Lu Frank W
Wells Sandra E.
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