Complementation assay for drug screening

Details Complementation assay for drug screening Complementation assay for drug screening

1993-07-21

1995-07-18

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 72, 435 78, 435 79, 436500, 436501, 436503, 436537, G01N 33535, G01N 33542, G01N 33566

Patent

active

054340527

ABSTRACT:
Compounds are evaluated for their binding to naturally occurring receptors, by employing the natural ligand conjugated to an enzyme donor fragment of .beta.-galactosidase for competing with the sample compound for the natural acceptor binding site or in the absence of competition where the sample compound binds to an allosteric site. By adding the enzyme acceptor fragment of the .beta.-galactosidase and substrate, the binding affinity of the sample compound may be evaluated as a measure of agonist or antagonist capability.

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