Complement assay method

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is a biological cell or cell fragment

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436 63, 436821, 435 4, 435 7, 435 18, 435 29, 435 32, 435253, 435259, 435810, 435849, C12Q 118, C12Q 134, C12N 106, C12N 120

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044927614

ABSTRACT:
A method of determining (1) the activity of complement, (2) the bacteriolytic activity of serum, or (3) the titer of antibody in a sample which comprises: (a) incubating complement-sensitive microorganisms containing an assayable intracellular component with the sample, and (b) detecting the assayable component released into the sample by lysis of the microorganisms.

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Fundenberg et al., "Basic and Clinical Immunology" 2nd edition, Lange Medical Publishers (1978), pp. 370-373, 220, and 600.
Yasuda et al., "A Simple Method to Measure Anti-Glycolipid Antibody by Using Complement-Mediated Immune Lysis of Fluorescent . . . " Journal of Immunological Methods 44 (9-1981), pp. 153-158.
D'Orazino et al., "Potentiometric Electrode Measurement of Serum Antibodies Based on the Complement Fixation Test" Analytica Chemica Acta 109 (1979), pp. 25-31.
Snyderman et al., "Deficiency of the Fifth Component of Complement in Human Subjects" American Journal of Medicine 67 (10-1979), pp. 638-645.
Pelczar et al., "Manual of Microbiological Methods" McGraw Hill Inc. NY, (1957), pp. 215-223.

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