Competitor primer asymmetric polymerase chain reaction

Details Competitor primer asymmetric polymerase chain reaction Competitor primer asymmetric polymerase chain reaction

1996-04-05

1997-05-06

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

536 243, C12P 1934

Patent

active

056270542

ABSTRACT:
A process for determining both the presence and number of copies of a target nucleic acid sequence in a sample being analyzed. In this process, amplification of a target nucleic acid sequence produces single-stranded DNA that is efficiently detected by nucleic acid probe hybridization and is present in an amount that is proportional to the number of copies of the target sequence originally present in a sample.

REFERENCES:
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Liu et al, Synthesis of a fixed-length single-stranded DNA modes by blocking primer extension in Bacteriophys MI3, Gene 1986, 42(1), 113-17.
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Samer Rifal et al, "Synthetic Exfoliative Toxin A and B DNA Probes for Detection of Toxigenic Staphylococcus aureus Strains", Journal of Clinical Microbiology, vol. 27, No. 3., Mar. 1989, pp. 504-506.
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