Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1988-04-12
1990-05-01
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 7, 435810, 436531, 436810, 935 77, 935 78, 422 61, C12Q 168, G01N 33535, G01N 33545
Patent
active
049217887
ABSTRACT:
A kit and a method for performing a competitive immunoassay utilize nucleic acid oligonucleotide chains for the detection of analytes, such as drugs, substances of abuse, hormones, poisons, organic compounds, peptides, proteins and the like. The kit includes a hapten-oligonucleotide complex, a complementary oligonculeotide chain for conjugating with the hapten-oligonucleotide complex, an antibody specific for the hapten and a flourescent label such as ethidium bromide having an affinity for nucleic acid duplexes formed from the hapten-oligonucleotide complex and the complementary oligonucleotide chain. A means for detecting the presence of the florescent label such as a U.V. transilluminator, U.V. lightbox and fluorescence spectrophotometer is utilized to detect color intensity and fluorescence of the dye. The method can be performed in solution, or on a solid "dipstick" on which the reagents for the immunoassay have been immobilized.
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Krupen Karen
Nucker Christine M.
The Research Foundation of State University of New York
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