Competitive immunofluorescence assay and test kit

Details Competitive immunofluorescence assay and test kit Competitive immunofluorescence assay and test kit

1983-05-27

1985-10-22

Nucker, Christine M.

Chemistry: analytical and immunological testing

Thyroid hormone tests

436540, 436800, 436808, 436815, 436817, 436826, 422 61, G01N 3352, G01N 3358, B65D 7100

Patent

active

045489084

ABSTRACT:
Competitive immunofluorescence assays for antigens in which immune complexes are precipitated with a nonfluorescent, nonlight-scattering precipitant such as polyethylene glycol and the resulting immunoprecipitate is dissolved with a nonfluorescent solvent of low ionic strength that maintains the pH of the solution substantially constant for immunofluorescence intensity reading. The assays are carried out by incubating the sample with fluorescent-labeled antigen, anti-antigen antibody, and a secondary antibody to the anti-antigen antibody followed by addition of the precipitant to form an immunoprecipitate. The precipitate is separated by centrifuging, dissolved in the solvent, and the immunofluorescence intensity of the solution is read with a fluorometer and compared to a standard curve.

REFERENCES:
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patent: 4279617 (1981-07-01), Masson et al.
patent: 4298592 (1981-11-01), Lin
patent: 4339427 (1982-07-01), Goldstein et al.
Pourfarzaneh et al., Clin. Chem. 26 (6) 730-731 (1980).
Chard et al., Clin. Chem., 25 (6) 973-976 (1979).
Cidosky et al., Analytical Chem. 51 (11) 1602-1605 (1979).
Karnes et al., Clin. Chem., 27 (1981) 249-252.
Soini et al., Clin. Chem., 25 (1979) 353-361.

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