Competitive immunoassay method, device and test kit

Details Competitive immunoassay method, device and test kit Competitive immunoassay method, device and test kit

1986-12-24

1989-02-07

Rosen, Sam

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436514, 436807, 436808, 436809, 422 61, 422 56, G01N 3353, G01N 33543

Patent

active

048031704

ABSTRACT:
An immunoassay device including one or more reaction chambers, each adapted to receive and retain a volume of test fluid in fluid communication with nonoverlapping first, second, and third reagent-bearing surfaces. To the first surface is reversibly bound an analyte conjugate: analyte component conjugated to one or more components, termed ligand/marker, that serve ligand and marker functions as described herein. Analyte binding partner is immobilized on the second surface, and ligand/marker binding partner is immobilized on the third surface. The reaction chamber is preferably configured to receive and direct the test fluid sequentially past the first second, and third reagent surfaces. In use, analyte conjugate solubilized from the first surface completes with any analyte in the test fluid for analyte binding partner sites on the second surface. Excess analyte conjugate becomes sequestered on the third surface, where the marker activity is read to indicate analyte presence and concentration in the test fluid. A test kit includes the immunoassay device in combination with comparative test results.

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