Competitive immunoassay device

Chemistry: analytical and immunological testing – Involving diffusion or migration of antigen or antibody

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436518, 436527, 436538, 436808, 436805, 436810, 422 56, 422 58, 422 61, 422 59, 435 71, 435 79, 435 793, 435975, G01N 33558, G01N 2100, G01N 3122

Patent

active

056482744

ABSTRACT:
A chromatographic assay device for detection and/or determination of an analyte in a competitive immunoassay gives a semiquantitative or quantitative indication of analyte concentration in a single assay device while also giving a positive indication that flow has occurred properly through the device. In one form, the device comprises: (1) a first opposable component including a sample preparation zone and an absorber; and (2) a second opposable component including a first chromatographic medium with capture and detection zones, a second chromatographic medium with a comparison zone, and a comparison label. In another form, the second opposable component includes one chromatographic medium with capture, detection, and control zones. Test kits incorporating the devices and methods for their use are also disclosed.

REFERENCES:
patent: 5395754 (1995-03-01), Lambotte et al.
patent: 5424220 (1995-06-01), Goerlach-Graw et al.
Wilchek, M. The Avidin-biotin complex in immunology, 1984; May 2; 39-43.

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