Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2007-10-16
2007-10-16
Le, Long V. (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S021000, C435S968000, C436S544000, C436S546000, C436S056000, C436S800000, C530S403000, C530S406000, C530S807000, C568S017000
Reexamination Certificate
active
10943463
ABSTRACT:
The present invention provides ligand-detection reagents, ligand analogs and methods for determining the presence of a ligand in a sample. The ligand-detection reagent comprises a ligand-binding antibody and a ligand analog to form an antibody-ligand analog complex wherein the ligand analog is covalently bonded to a reporter molecule. This complex may additionally comprise a labeling protein non-covalently bonded to the antibody to form a ternary complex wherein the labeling protein comprises a monovalent antibody fragment or a non-antibody protein that is covalently bonded to a label moiety. The reporter molecule is either quenched by the ligand-binding antibody or by the label moiety of the labeling protein, depending on the reporter molecule and the ligand-binding antibody, wherein the amount of quenching is directly related to the amount of ligand present in the sample. Alternatively, the ligand analog is fluorogenic wherein the ligand analog is essentially non-fluorescent in solution but when bound by the ligand-binding antibody the detectable signal increases. In this instance a decrease in signal, as opposed to the relieving of quenching, is measured for the presence of a target ligand.
REFERENCES:
patent: 3996345 (1976-12-01), Ullman et al.
patent: 3998943 (1976-12-01), Ullman
patent: 4160016 (1979-07-01), Ullman
patent: 4174384 (1979-11-01), Ullman et al.
patent: 4196265 (1980-04-01), Koprowski et al.
patent: 4261968 (1981-04-01), Ullman et al.
patent: 4318846 (1982-03-01), Khana et al.
patent: 4603209 (1986-07-01), Tsien et al.
patent: 4714763 (1987-12-01), Theodoropulos
patent: 4774339 (1988-09-01), Haugland et al.
patent: 4810636 (1989-03-01), Corey
patent: 4812409 (1989-03-01), Babb et al.
patent: 4849362 (1989-07-01), DeMarinis et al.
patent: 4945171 (1990-07-01), Haugland et al.
patent: 4981977 (1991-01-01), Southwick et al.
patent: 5132432 (1992-07-01), Haugland et al.
patent: 5187288 (1993-02-01), Kang et al.
patent: 5227487 (1993-07-01), Haugland et al.
patent: 5242805 (1993-09-01), Naleway et al.
patent: 5248782 (1993-09-01), Haugland et al.
patent: 5268486 (1993-12-01), Wagonner et al.
patent: 5274113 (1993-12-01), Kang et al.
patent: 5360895 (1994-11-01), Hainfeld et al.
patent: 5433896 (1995-07-01), Kang et al.
patent: 5442045 (1995-08-01), Haugland et al.
patent: 5451343 (1995-09-01), Neckers et al.
patent: 5459276 (1995-10-01), Kuhn et al.
patent: 5486616 (1996-01-01), Waggoner et al.
patent: 5501980 (1996-03-01), Katerinopoulos et al.
patent: 5569587 (1996-10-01), Waggoner
patent: 5569766 (1996-10-01), Waggoner et al.
patent: 5627027 (1997-05-01), Waggoner
patent: 5696157 (1997-12-01), Wang
patent: 5798276 (1998-08-01), Haugland et al.
patent: 5808044 (1998-09-01), Brush et al.
patent: 5830912 (1998-11-01), Gee et al.
patent: 5846737 (1998-12-01), Kang
patent: 5877310 (1999-03-01), Reddington et al.
patent: 6002003 (1999-12-01), Shen et al.
patent: 6080868 (2000-06-01), Lee et al.
patent: 6130101 (2000-10-01), Mao et al.
patent: 6162931 (2000-12-01), Gee et al.
patent: 6229055 (2001-05-01), Klaubert et al.
patent: 6348596 (2002-02-01), Lee et al.
patent: 6399392 (2002-06-01), Haugland et al.
patent: 6441140 (2002-08-01), Comb et al.
patent: 6541618 (2003-04-01), Lee et al.
patent: 1 065 250 (2001-01-01), None
patent: WO97/40104 (1997-10-01), None
patent: WO99/51702 (1999-10-01), None
patent: WO 01/21624 (2001-03-01), None
patent: WO 02/26891 (2002-04-01), None
patent: WO 03/030817 (2003-04-01), None
Lee et al. (1999): STN international HCAPLUS database, columbus (OH), accession No. 1999: 120261.
Zhang et al. (2003): STN international HCAPLUS database, columbus (OH), accession No. 2003: 396733.
Zhang, J. H., T. D. Chung, et al. (1999). “A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays.”J Biomol Screen4(2): 67-73.
Tyagi, S., D. P. Bratu, et al. (1998). “Multicolor molecular beacons for allele discrimination.”Nat Biotechnol16(1): 49-53.
Tyagi, S. and F. R. Kramer (1996). “Molecular beacons: probes that fluoresce upon hybridization.”Nat Biotechnol14(3): 303-8.
dos Remedios, C. G. and P. D. Moens (1995). “Fluorescence resonance energy transfer spectroscopy is a reliable “ruler” for measuring structural changes in proteins. Dispelling the problem of the unknown orientation factor.”J Struct Biol115(2): 175-85.
Wu, P. and L. Brand (1994). “Resonance energy transfer: methods and applications.”Anal Biochem218(1): 1-13.
Selvin, P. R. (1995). “Fluorescence resonance energy transfer.”Methods Enzymol246: 300-34.
Matayoshi, E. D., G. T. Wang, et al. (1990). “Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transfer.”Science247(4945): 954-8.
Gorevic, P. D., F. C. Prelli, et al. (1985). “Immunoglobulin G (lgG).”Methods Enzymol116: 3-25.
Morrison, L.E. (1992). “Detection of Energy Transfer and Fluorescence Quenching.”Nonisotopic DNA Probes Techniques311-52.
Furniss, B. S., A. J. Hannaford, et al. (1989).Vogel's Textbook of Practical Organic Chemistry5thEd: 809-23.
Heller, A. (1990). “Electrical Wiring of Redox Enzymes.” Acc Chem Res 23: 128-34.
Haugland, R. P. (2002).Handbook of Fluorescent Probes and Research Products9thEd.
Beechem Joseph
Gee Kyle Richard
Hagen David Carl
Johnson Iain D
Kang Hee-chol
Haq Shafiqul
Invitrogen Corporation
Le Long V.
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