Competitive hybridization technique

Details Competitive hybridization technique Competitive hybridization technique

1996-02-28

1998-10-20

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 243, 935 77, 935 78, C12Q 168, C07H 2102, C07H 2104

Patent

active

058244760

ABSTRACT:
Discrimination between RNAs which differ by as little as a single nucleotide is accomplished by having an excess of unlabeled non-complementary oligonucleotide present during hybridization with a labeled complementary nucleotide. The non-complementary oligonucleotide blocks hybridization of the labeled complementary oligonucleotide sequence without affecting hybridization of the labeled complementary oligonucleotide to the complementary sequence. The label may be an isotope, a fluorescent group or of any other type. The technique permits examination of the transcription of highly related allelic and non-allelic genes present in the same cell and the quantification of such transcripts by use of appropriate internal control. The technique is also useful in oligonucleotide hybridization to DNA sequences which differ only by a single nucleotide.

REFERENCES:
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Conner et al., Proc. Natl. Acad. Sci. USA, 80:278-282 (1983).
Studencki & Wallace, DNA 3(1):7-15 (1984).

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