Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1977-07-29
1980-11-25
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
424 12, 435 28, 435177, G01N 3114
Patent
active
042359600
ABSTRACT:
A competitive enzyme-linked immunoassay for a soluble analyte in which the analyte in the unknown sample and the same analyte attached to a solid phase (immunosorbent) compete for a selected quantity of a soluble first antibody to the substance; a second or bridging antibody is then linked to any first antibody linked to the immunosorbent as a result of the competitive reaction, following which a soluble enzyme/antibody immune complex is linked to bridging antibody on the immunosorbent. The enzymatic activity associated with the thusly-formed enzyme-immunosorbent complex or with the non-immunosorbent fraction of enzyme is measured to determine the presence and/or concentration of analyte in the unknown sample.
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patent: 4002532 (1977-01-01), Weltman et al.
patent: 4021534 (1977-05-01), Lafontaine
patent: 4024235 (1977-05-01), Weetall et al.
patent: 4034074 (1977-07-01), Niles
patent: 4048298 (1977-09-01), Niswender
Yorde, et al., Competitive Enzyme-Linked Immunoassay with Use of Soluble Enzyme/Antibody Immune, Complexes For Labeling I. Measurement of Human Choriogonadotropin. Clinical Chemistry vol. 22, No. 8 1976 (pp. 1372-1377).
Hinton, et al., The Unlabeled Antibody Enzyme Method of Immunohistochemistry. The Journal of Histochemistry and Cytochemistry, vol. 21, No. 11, 1973 (pp. 978-998).
Sasse Edward A.
Yorde Donald E.
Naff David M.
The Medical College of Wisconsin Inc.
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