Competitive binding analysis by fluorescence

Details Competitive binding analysis by fluorescence Competitive binding analysis by fluorescence

1977-09-01

1981-06-09

Fagelson, Anna P.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 250302, 424 1, 424 11, 424 12, 424 13, G01N 3378, G01N 3358, G01N 3354, G01N 2164

Patent

active

042725054

ABSTRACT:
Thyroid hormones such as thyroxine and triiodothyronine are assayed in biological fluid samples by a competitive binding analysis in which a fluorescent label is used. Upon formation of complexes, the fluorescence of the labelled compound is released, and the hormone can be assayed by measuring the fluorescence of the mixture and comparing the result with standard data. Fluorescein is the preferred label. The process can be operated in a continuous flow manner.

REFERENCES:
patent: 3901654 (1975-08-01), Gross
patent: 3940475 (1976-02-01), Gross
patent: 3973129 (1976-08-01), Blumberg
patent: 3992631 (1976-11-01), Harte
patent: 4061466 (1977-12-01), Sjoholm
Nilsson et al., J. Biol. Chem., vol. 250, No. 21, Nov. 1975, pp. 8554-8563.
Williams et al., Methods in Immunol. & Immuno-Chem. Acadamic Press, NY, vol. III, 1971, pp. 427-443.
Technicon, 1972 Technicon Int. Cong., Advances in Automated Analysis, vol. 4, 1973, pp. 87-91.

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