Competitive assays using chemiluminescent electron-rich aryl-sub

Details Competitive assays using chemiluminescent electron-rich aryl-sub Competitive assays using chemiluminescent electron-rich aryl-sub

1995-05-09

1998-07-14

Achutamurthy, Ponnathapura

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 4, 435 6, 435 71, 435 79, G01N 3353, G01N 33543

Patent

active

057802491

ABSTRACT:
Chemiluminescent electron-rich aryl-substituted 1,2-dioxetane compounds are disclosed in which the aryl group is poly-substituted with suitable electron-donating groups such that the light-emitting pattern of the molecule results in a very high luminescent count, thus providing for a sensitive and precise assay for haptens, analytes, polynucleotides and the like. These substituted aryl-containing 1,2-dioxetane compounds can be used as direct labels in an immunoassay or when derivatized with an appropriate leaving group, can be used as a substrate for a enzyme immunoassay. The unusual chemiluminescence of the compounds allows the timing of the luminescent reaction to be exactly controlled.

REFERENCES:
patent: 4931569 (1990-06-01), Edwards et al.
patent: 5004565 (1991-04-01), Schaap
patent: 5112960 (1992-05-01), Bronstein et al.

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