Comparative genomic hybridization

Details Comparative genomic hybridization Comparative genomic hybridization

2007-07-03

2007-07-03

Fredman, Jeffrey (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

Reexamination Certificate

active

11017493

ABSTRACT:
Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).

REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4647529 (1987-03-01), Rodland et al.
patent: 4666828 (1987-05-01), Gusella et al.
patent: 4681840 (1987-07-01), Stephenson et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4707440 (1987-11-01), Stavianopoulos
patent: 4710465 (1987-12-01), Weissman et al.
patent: 4711955 (1987-12-01), Ward et al.
patent: 4721669 (1988-01-01), Barton
patent: 4725536 (1988-02-01), Fritsch et al.
patent: 4770992 (1988-09-01), Van den Engh et al.
patent: 4772691 (1988-09-01), Herman
patent: 4888278 (1989-12-01), Singer et al.
patent: 4981783 (1991-01-01), Augenlicht
patent: 5085983 (1992-02-01), Scanlon
patent: 5194300 (1993-03-01), Cheung
patent: 5348855 (1994-09-01), Dattagupta
patent: 5427932 (1995-06-01), Weier et al.
patent: 5447841 (1995-09-01), Gray et al.
patent: 5472842 (1995-12-01), Stokke et al.
patent: 5665549 (1997-09-01), Pinkel et al.
patent: 5721098 (1998-02-01), Pinkel et al.
patent: 5800992 (1998-09-01), Fodor et al.
patent: 5830645 (1998-11-01), Pinkel et al.
patent: 5840484 (1998-11-01), Seilhamer et al.
patent: 5856097 (1999-01-01), Pinkel et al.
patent: 5965362 (1999-10-01), Pinkel et al.
patent: 5976790 (1999-11-01), Pinkel et al.
patent: 6159685 (2000-12-01), Pinkel et al.
patent: 6335167 (2002-01-01), Pinkel et al.
patent: 2024769 (1991-03-01), None
patent: 0237362 (1987-09-01), None
patent: 0326989 (1989-08-01), None
patent: 0430402 (1991-06-01), None
patent: 2019408 (1979-10-01), None
patent: 2215724 (1989-09-01), None
patent: 87/05027 (1987-08-01), None
patent: 90/05789 (1990-05-01), None
f http://www.ndsu.nodak.edu/instruct/mcclean/plsc431/eukarychrom/eukaryo3.htm (accessed Mar. 10, 2006).
Becher et al (Cancer Research (1983) 43:5010-5016).
Trask et al (Trends in Genetics (1991) 7(5):149-154).
Albertson, “Localization of the Ribosomal Genes inCaenorhabditis elegansChromosomes byin situHybridization Using Biotin-Labeled Probes,”EMBO J.3:6:1227-1234 (1984).
Albertson, “Mapping Muscle Protein Genes byin situHybridization Using Biotin-Labeled Probes,”EMBO J.4:10:2493-2498 (1985).
Angerer et al., “In SituHybridization to Cellular RNAs,”Genetic Engineering: Principles and MethodsSetlow and Hollaender, Eds., Plenum Press, N.Y., 7:43-65 (1985).
Ardeshir et al., “Structure of Amplified DNA in Different Syrian Hamster Cell Lines Resistant to N-(Phosphonacetyl)-L-Aspartate,”Molecular and Cellular Biology3:11:2076-2088 (Nov. 1983).
Arnoldus et al., “Detection of the Philadelphia Chromosome in Interphase Nuclei (With 2 Color Plates),”Cytogenet. Cell Genet.54:108-111 (1990).
Bar-Am et al., “Detection of Amplified DNA Sequences in Human Tumor Cell Lines by Fluorescence in situ Hybridization,”Genes, Chromosomes&Cancer4:314-320 (1992).
Bayer et al., “The Use of the Avidin-Biotin Complex as a Tool in Molecular Biology,”Methods of Biochemical Analysis26:1-45 (1980).
Benton et al., “Screening λgt Recombinant Clones by Hybridization to Single Plaquesin situ,” Science196:180-182 (1977).
Bergerheim et al., “Deletion Mapping in Human Renal Cell Carcinoma,”Cancer Res.49:1390-1396 (Mar. 1989).
Bookstein et al., “Human Retinoblastoma Susceptibility Gene: Genomic Organization and Analysis of Heterozygous Intragenic Deletion Mutants,”PNAS(USA) 85:2210-2214 (Apr. 1988).
Brigati et al., “Detection of Viral Genomes in Cultured Cells and Paraffin-Embedded Tissue Sections Using Biotin-Labeled Hybridization Probes,”Virology126:32-50 (1983).
Brison et al., “General Method for Cloning Amplified DNA by Differential Screening With Genomic Probes,”Molecular and Cellular Biology2:5:578-587 (May 1982).
Britten et al., “Analysis of Repeating DNA Sequences by Reassociation,”Methods of Enzymology29:363-418 (1974).
Brock et al., “Quantitative in situ Hybridization Reveals Extent of Sequence Homology Between Related DNA Sequence inDrosphilia melanogaster,” Chromosoma83:159-168 (1981).
Broker et al., “Electron Microscopic Visualization of tRNA Genes with Ferritin-Avidin: Biotin Labels,”Nucleic Acids Research5:2:363-384 (1978).
Bufton, et al., “A Highly Polymorphic Locus on Chromosome 16q Revealed by a Probe from a Chromosome-Specific Cosmid Library,”Human Genetics74:425-431 (1986).
Bufton et al., “Four Restriction Fragment Length Polymorphisms Revealed by Probes From a Single Cosmid Map to Human Chromosome 19,”Am. J. Hum. Genet.38:447-460 (1986).
Buongiorno-Nardelli et al., “Autoradiographic Detection of Molecular Hybrids between RNA and DNA in Tissue Sections,”Nature225:946-948 (Mar. 1970).
Burk et al., “Organization and Chromosomal Specificity of Autosomal Homologs of Human Y Chromosome Repeated DNA,”Chromosoma92:225-233 (1985).
Buroker et al., “Four Restriction Fragment Length Polymorphisms Revealed By Probes From a Single Cosmid Map to Human Chromosome 12q,”Human Genetics72:86-94 (1986).
Cannizzaro et al., “In Situ Hybridization and Translocation Breakpoint Mapping II. Two Unusual t(21;22) Translocations,”Cytogenet. Cell Genet.39:173-178 (1985).
Cantor et al., “The Behavior of Biological Macromolecules, Part III,”Biophysical Chemistry, Freeman & Co. pp. 1183, 1226-1228 (1980).
Cohen et al., “Hereditary Renal-Cell Carcinoma Associated With a Chromosomal Translocation,”N. Engl. J. Med.301:11:592-595 (Sep. 1979).
Collins and Weissman, “Directional cloning of DNA fragments at a large distance from an initial probe: A circularization method,”PNAS(USA) 81:6812-6816 (Nov. 1984).
Connolly et al., “Chemical Synthesis of Oligonucleotides Containing a Free Sulphydryl Group and Subsequent Attachment of Thiol Specific Probes,”Nucleic Acids Research13:12:4485-4502 (1985).
Cote et al., “Quantitation of in situ Hybridization of Ribosomal Ribonucleic Acids to Human Diploid Cells,”Chromosoma80:349-367 (1980).
Cox et al., “Detection of mRNAs in Sea Urchin Embryos byin situHybridization Using Asymmetric RNA Probes,”Developmental Biology101:485-502 (1984).
Cremer et al., “Preparative Dual-Beam Sorting of the Human Y Chromosome and In Situ Hybridization of Cloned DNA Probes,”Cytometry-5:572-579 (1984).
Cremer et al., “Detection of Chromosome Aberrations in the Human Interphase Nucleus by Visualization of Specific Target DNAs with Radioactive and Non-radioactive in situ Hybridization Techniques: Diagnosis of Trisomy 18 with Probe L1.84,”Hum. Genetics74:346-352 (1986).
Cremer et al., “Rapid Interphase and Metaphase Assessment of Specific Chromosomal Changes in Neuroectodermal Tumor Cells by In Situ Hybrid

Affiliated with

Also associated with

Rating

Comparative genomic hybridization does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Comparative genomic hybridization, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Comparative genomic hybridization will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3772107