Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1984-07-16
1987-03-24
Cohan, Alan
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435291, 435808, 422 57, 422 81, 436527, 436805, G01N 3358
Patent
active
046525203
ABSTRACT:
A method and device for performing comparative assay of binding and bindable substances such as antibodies and antigens. The device includes a circulation conduit for continuously conveying a carrier fluid between a support zone therein and a detection zone therein. A competitive binding substance is fixed in the support zone and is capable of competitively binding a test substance and an analytical detection substance such that changes in concentration of the test substance in the carrier fluid responsively cause changes of concentration of analytical detection substance in the carrier fluid. Each of the analytical detection substance (antigen-fluorochrome, for example) and the test substance (antigen, for example) are bound to the binding substance (monoclonal antibody, for example) in proportion to the concentration of the test substance in the carrier fluid. A detection means is provided for detecting changes in the concentration of the analytical detection substance as the carrier fluid is conveyed through the detection zone. Preferably, the detection means comprises a fluorescent detector detecting fluorescent activity produced by the fluorochrome detection substance. The fluorescent detector includes a comparator which compares the degree of fluorescent activity in a lo-flow tube compared to a hi-flow tube. Changes in concentration of the test substance produce a variation in the fluorescent activity in the hi-flow tube compared to the lo-flow tube. The method includes introducing a test substance into the carrier fluid of a device of the type described above. A comparative assay is performed by detecting changes in the concentration of the analytical detection substance produced by the introduction of the test substance.
REFERENCES:
patent: 3838011 (1974-09-01), Hagen
patent: 3973911 (1976-08-01), Swolinski
patent: 4425438 (1984-01-01), Bauman
Cohan Alan
Immuno-Mycologics, Inc.
LandOfFree
Comparative assay method and device does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Comparative assay method and device, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Comparative assay method and device will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1353811