Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...
Reexamination Certificate
2006-05-23
2006-05-23
Kallis, Russell P. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide contains a tissue, organ, or cell...
C278S021000, C278S021000, C278S021000, C435S419000, C435S069100
Reexamination Certificate
active
07049484
ABSTRACT:
Production of proteases in plants is set forth, whereby heterologous DNA encoding the protease is introduced into the plant and expression of the protein achieved. By such methods, expression is achieved in plants wherein the plant cell is not damaged, the protein can be recovered without contamination by other proteases, and can be expressed at levels such that commercial production of the enzyme is obtained. Expression levels can be at 0.1% of total soluble protein of the plant, or higher.
REFERENCES:
patent: 5097025 (1992-03-01), Benfey et al.
patent: 5122594 (1992-06-01), Yoshida et al.
patent: 5215912 (1993-06-01), Hoffman
patent: 5270200 (1993-12-01), Sun et al.
patent: 5290924 (1994-03-01), Last et al.
patent: 5290927 (1994-03-01), Last et al.
patent: 5358934 (1994-10-01), Borovsky et al.
patent: 5424200 (1995-06-01), Mc Pherson et al.
patent: 5457066 (1995-10-01), Frank et al.
patent: 5504200 (1996-04-01), Hall et al.
patent: 5543501 (1996-08-01), Slingtom et al.
patent: 5552527 (1996-09-01), Godiard et al.
patent: 5629196 (1997-05-01), Borovsky et al.
patent: 5693520 (1997-12-01), Branner et al.
patent: 5714580 (1998-02-01), Brunck et al.
patent: 5741664 (1998-04-01), Ballinger et al.
patent: 265 164 (1989-02-01), None
patent: 265164 (1989-02-01), None
patent: 0597681 (1994-05-01), None
patent: WO 92/01042 (1992-01-01), None
patent: WO9201042 (1992-01-01), None
patent: WO 95/14099 (1995-05-01), None
patent: WO9414099 (1995-05-01), None
patent: WO 96 21033 (1996-07-01), None
patent: WO9621033 (1996-07-01), None
patent: WO 98/36085 (1998-08-01), None
patent: WO9836085 (1998-08-01), None
patent: WO0114571 (2001-03-01), None
Christensen et al, Plant Mol. Biol., vol. 18, pp. 675-689, 1992.
Odell et al, Nature, vol. 313, pp. 810-812, 1985.
Rogers et al, J. Biol. Chem., vol. 258, pp. 8169-8174, 1983.
Schroeder et al, J. Biol. Chem., vol. 243, pp. 2943-2949, 1968.
Altenbach et al, Plant Mol. Biol., vol. 18, pp. 235-245, 1992.
Wim J. M. Van de Wen,et al., “Structure and Function of Eukaryotic Proprotein Processing Enzymes of the Subtilisin Family of Serine Proteases”,Critical Reviews in Oncogenesis4 (2) 115-136 1993.
Takagi,Hiroshi,et al., “Enhancement of the Thermostability of Subtilisin E by Introduction of a Disulfide Bond Engineered on the Basis of Structural Comparison with a Thermophilic Serine Protease”,The Journal of Biological Chemistryvol. 265,No. 12 pp. 6874-6878, Apr. 25, 1990.
Svendsen,I. B, “Chemical Modifications of The Subtilisins With Special Reference to the Binding of Large Substrates. A Review”, Carlsberg Res. Commun. vol. 41,No. 5, 1976.
Pierce,Jeffrey A., et al., “Physiological and Genetic Strategies for Enhanced Subtilisin Production byBacillus subtilis”, Biotechnol. Prog., vol. 8,pp. 211-218, 1992.
Wells, James A.,et al., “Subtilisin-an enzyme designed to be engineered”,Trends in Biochemical Sciences, Aug. 1988.
Hiei, Yukoh, et al., “Efficient transformation of rice (Oryza sative L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA”,The Plant Journal6 (2), pp 271-282 Jan. 1994.
Ishida, Y. et al. “High efficiency transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA” The Plant Journal 6 (2), pp. 271-282, Jan. 1994.
Cheng,Ming, et al., “Genetic Transformation of Wheat Mediated by Agrobacterium tumefaciens”,Plant Physiolpp. 971, 1997.
Bustos, Mauricio M., “Regualtion of B-Glucuronidase Expression in Transgenic Tobacco Plants by an A/T-Rich, cis-Acting Sequence Found Upstream of a French Bean B-Phaseolin Gene”,The Plant Cell, vol. 1, Sep. 1989.
Christensen et al, “Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation” Plant Mol. Biol., vol. 18, pp 675-689, 1992.
Odel et al., “Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter” Nature, vol. 313, pp 810-812 1985.
Rogers et al., “Isolation and sequence analysis of a barley α-amylase cDNA clone” J. Biol. Chem.., vol., 258, pp 8169-8174, 1983.
Shroeder et al., J. Biol. Che., vol. 243, pp 2943-2949, 1968.
Moon, E. “In situ transient expression of theE. coliβ-glucuronidase modified at the glycosylation Site” Mol. Cell, vol. 6, No. 1, pp 56-60.
Altenbach et al., “Accumulation of a brazil nut albumin in seeds of transgenic canola results in enhanced levels of seed protein methionine” Plant Mol. Biol, vol. 18, pp 235-245, 1992.
van der Geest, Al., and Hall, T. “A 68 bp element of the beta-phaseolin promoter functions as a seed-specific enhancer” Plant Molecular Biology, 32:579-588, 1996.
Wyatt, Paul et al. “Corrrect processing of the kiwifruit protease actinidin in transgenic tobacco requires the presence of the C-terminal propeptide” Plant Physiol. 108(1) 261-8, 1995.
Wim J. M. Van de Wen,et al., “Structure and Function of Eukaryotic Proprotein Processing Enzymes of the Subtilisin Family Proteases”,Critical Reviews in Oncogenesis4 (2) 115-136 1993.
Takagi,Hiroshi,et al., “Enhancement of the Thermostability of Subtilisin E by Introduction of a Disulfide Bond Engineered on the Basis of Structural Comparison with a Thermophilic Serine Protease”,The Journal of Biological Chemistryvol. 265,No. 12 pp. 6874-6878, Apr. 25, 1990.
Svendsen,I. B, “Chemical Modifications of The Subtilisins With Special Reference to the Binding of Large Substrates. A Review”, Carlsberg Res. Commun. vol. 41,No. 5, 1976.
Pierce,Jeffrey A., et al., “Physiological and Genetic Strategies for Enhanced Subtilisin Production byBacillus subtilis”, Biotechnol. Prog., vol. 8,pp. 211-218, 1992.
Wyatt, Paul et al. “Correct processing of the kiwifruit protease actinidin in transgenic tobacco requires the presence of the C-terminal propeptide”Plant Physiol.108(1), 261-8 (1995).
Hood Elizabeth
Howard John A.
Kallis Russell P.
Prodi Gene, Inc.
Sweeney Patricia A.
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